HEK-Blue™ TGF-β Cells

TGF-β Sensor Cells

HEK-Blue™ TGF-β cells allow the detection of bioactive TGF-β by monitoring the activation of the TGF-β/Smad pathway. They were generated by stable transfection of HEK293 cells with the human TGFBRI, Smad3 and Smad4 genes. They further express a Smad3/4-binding elements (SBE)-inducible SEAP reporter gene. Stimulation of HEK-Blue™ TGF-β cells with TGF-β induces the activation of the TGF-β/Smad signaling pathway leading to the formation of a Smad3/Smad4 complex. The heterocomplex enters the nucleus and binds SBE sites inducing the production of SEAP. The quantity of SEAP secreted in the supernatant can be easily assessed using QUANTI-Blue™. TGF-β-mediated SEAP production can be blocked using a neutralizing antibody, such as anti-hTGFβ-IgA.

Figures for this product

Response of HEK-Blue™ TGF-β cells to TGF-βCytokine response profile of HEK-Blue™ TGF-β cellsNeutralization of TGF-β response


Antibiotic resistance: blasticidin, hygromycin B, Zeocin™

Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™

Guaranteed mycoplasma-free

Shipped on dry ice

Detects human TGF-β
Detection range for human TGF-β: 0.1 - 10 ng/ml


• 1 vial containing 3-7 x 106 cells

• 100 μl Blasticidin (10 mg/ml)

• 100 μl Hygromycin B Gold (100 mg/ml)

• 100 μl Zeocin™ (100 mg/ml)

• 1 ml Normocin™ (50 mg/ml)

• 1 pouch of QUANTI-Blue™ (SEAP detection medium)


HEK-Blue TGF-beta pathway

Tumor growth factor-beta (TGF-β) belongs to a family of structurally related cytokines that regulate a plethora of cellular functions, such as proliferation, apoptosis, differentiation and migration.

TGF-β binds to a type II receptor which recruits and activates a type I receptor. The type I receptor then phosphorylates receptor-regulated Smads (R-Smads), such as Smad2 and Smad3, which associate with Smad4. R-Smad/Smad4 complexes accumulate in the nucleus where they regulate the transcription of target genes.

HEK-Blue™ TGF-β cells are designed to monitor TGF-β-induced Smad signaling, as these cells express a Smad3/4-binding elements (SBE)-inducible SEAP reporter gene.


Recent articles using InvivoGen HEK-Blue™ TGF-β Cells


HEK-Blue™ TGF-β cells

Cat. Codehkb-tgfb
Unit Size3-7 x 10e6 cells
Price For price or distributor address,
please select your country
Disclaimer: Our products are provided for research purpose only. Commercial applications may require licensing from third parties.
Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
Copyrights © 2011-2016 InvivoGen. All Rights Reserved. Reproduction of any materials from this site is strictly forbidden without permission for commercial use. Nonprofit use for non-commercial research and educational purposes is permitted, citation should include the URL "www.invivogen.com".