SEAP Reporter System
Secreted embryonic alkaline phosphatase (SEAP) is a reporter widely used to study promoter activity or gene expression.
It is a truncated form of human placental alkaline phosphatase (PLAP) by deletion of the GPI anchor.
Unlike endogenous alkaline phosphatases, PLAP is extremely heat stable and resistant to the inhibitor L-homoarginine. SEAP is secreted into cell culture supernatant and therefore offers many advantages over intracellular reporters.
It allows to determine reporter activity without disturbing the cells, does not require the preparation of cell lysates and can be used for kinetic studies.