|pUNO1-hRIPK3||Unit size||Cat. code||Docs||Qty||Price|
pUNO1 bearing the human RIPK3 gene
|pUNO1-mRIPK3a||Unit size||Cat. code||Docs||Qty||Price|
pUNO1 bearing the mouse RIPK3 gene, isoform 1
Expression-ready ORF Clones
Alias : RIP3
Receptor interacting serine/threonine kinase 3.
Receptor-interacting protein 3 (RIP3) is a member of the RIP Ser/Thr kinase family . Unlike RIP1 and RIP2, RIP3 possesses neither a death domain nor a CARD motif at its unique C terminus . RIP3 is a potent inducer of apoptosis, an activity readily localizable to its C terminus. RIP3 binds RIP within the TNFR-1 signaling complex and attenuates its ability to engage the NF-kB pathway [2,3]. Upon TNF stimulation, RIP1 is recruited to the TNFR1 signaling complex to activate NF-kB. RIP3 is then recruited and phosphorylates RIP1 inhibiting its ability to activate NF-kB. Thus RIP3 acts as a modulator that can limit persistent activation of the NF-kB pathway .
1. Yu PW. et al., 1999. Identification of RIP3, a RIP-like kinase that activates apoptosis and NFkappaB. Curr Biol. 9(10):539-42.
2. Sun X. et al., 1999. RIP3, a novel apoptosis-inducing kinase. J Biol Chem. 274(24):16871-5.
3.Sun X. et al., 2002. Identification of a novel homotypic interaction motif required for the phosphorylation of receptor-interacting protein (RIP) by RIP3. J Biol Chem. 277(11):9505-11.
Human RIPK3 (pUNO1-hRIPK3)
Genbank : NM_006871.3
ORF size : 1557 bp
Subclone : BspLU11I - NheI
Mouse RIPK3 isoform 1 (pUNO1-mRIPK3a)
Genbank : NM_019955.2 with silent variation in codon Val291.
ORF size : 1461 bp
Subclone : AgeI - EcoRI
- 20 µg of lyophilized DNA
- 2 x 1 ml blasticidin at 10 mg/ml
Product is shipped at room temperature.
Lyophilized DNA should be stored at -20 ̊C.
Resuspended DNA should be stored at -20 ̊C and is stable up to 1 year.
Blasticidin is a harmful compound. Refer to safety data sheet for handling instructions.
Store blasticidin at 4°C or -20°C for up to two years. Product is stable 2 weeks at 37°C.
Avoid repeated freeze-thaw cycles.Back to the top