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RIG-I (DDX58)

pUNO1-hRIGI Unit size Cat. code Docs Qty Price
pUNO1 bearing the human RIG-I gene
20 µg
puno1-hrigi
+-
$384.00
pUNO1-hRIGI-DN Unit size Cat. code Docs Qty Price
pUNO1 bearing a dominant negative version of the human RIG-I gene
20 µg
puno1-hrigi-dn
+-
$406.00
pUNO1-mRIGI Unit size Cat. code Docs Qty Price
pUNO1 bearing the mouse RIG-I gene
20 µg
puno1-mrigi
+-
$384.00

Expression-ready ORF Clones

Alias : DDX58

 

RNA helicase retinoic acid inducible gene I.
DEAD [Asp-Glu-Ala-Asp] box polypeptide 58.

 

Available versions:

  • Wild type RIG-I
  • RIG-I DN: Dominant negative RIG-I (also called RIG-IC)
    Dominant negative RIG-I (RIG-IC) was generated by removing the two caspase recruitment domains (CARD) found at the N terminus (aa1-217). Expression of RIG-IC in L929 cells was shown to inhibit virus-induced gene expression [1].

 

References

1. Yoneyama M. et al., 2005. Shared and Unique Functions of the DExD/H-Box Helicases RIG-I, MDA5, and LGP2 in Antiviral Innate Immunity. J. Immunol. 175:2851-58.

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Specifications

Human RIG-I (pUNO1-hRIGI)

Genbank : NM_014314.3
ORF size : 2778 bp
Subclone : AgeI - NheI

Human RIG-I-DN (pUNO1-hRIGI-DN)

ORF size : 2130 bp
Subclone : AgeI - NheI

Mouse RIG-I (pUNO1-mRIGI)

ORF size : 2781 bp
Subclone : BspHI - XbaI

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Contents pUNO1

  • 20 µg of lyophilized DNA
  • 2 x 1 ml blasticidin at 10 mg/ml

 

room temperature Product is shipped at room temperature.

store Lyophilized DNA should be stored at -20 ̊C.

stability Resuspended DNA should be stored at -20 ̊C and is stable up to 1 year.

Alert Blasticidin is a harmful compound. Refer to safety data sheet for handling instructions.

 

Store blasticidin at 4°C or -20°C for up to two years. Product is stable 2 weeks at 37°C.

Avoid repeated freeze-thaw cycles.

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Citations

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