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IRAK1

pUNO1-hIRAK1a Unit size Cat. code Docs Qty Price
pUNO1 bearing the human IRAK1 gene, isoform 1
20 µg
puno1-hirak1
+-
$384.00
pUNO1-hIRAK1-DN Unit size Cat. code Docs Qty Price
pUNO1 bearing a dominant negative version of the human IRAK1 gene
20 µg
puno1-hirak1-dn
+-
$406.00
pUNO1-mIRAK1d Unit size Cat. code Docs Qty Price
pUNO1 bearing the mouse IRAK1 gene, isoform 4
20 µg
puno1-mirak1
+-
$384.00

Expression-ready ORF Clones

Alias : IRAK, pelle

 

Interleukin-1 receptor-associated kinase 1.

 

Available versions:

  • Wild type IRAK1
  • Dominant negative IRAK1 (see the "Details" tab)

Interleukin-1 receptor-associated kinases (IRAKs) are serine/threonine kinases that act as signal transduction mediators for the TLRs. The IRAK family consists of two active kinases, IRAK-1 and IRAK-4, and two inactive kinases, IRAK-2 and IRAK-M [1]. Upon stimulation, the adaptor MyD88 recruits IRAK-1, IRAK-2 and IRAK-4 to the receptor. IRAK-4 then phosphorylates IRAK-1 which dissociates from the receptor and interacts directly with TRAF-6 to relay the signal [2]. Subsequently, IRAK-1 undergoes autophosphorylation followed by degradation. IRAK-4 is an essential component of the IL-1, IL-18 and TLR signaling cascade [2-4].
 

References:

1. Janssens S. & Beyaert R. , 2003. Functional diversity and regulation of different interleukin-1 receptor-associated kinase (IRAK) family members. Mol Cell. 11(2):293-302.
2. Li S. et al., 2002. IRAK-4: a novel member of the IRAK family with the properties of an IRAK-kinase.Proc Natl Acad Sci U S A. 99(8):5567-72.
3. Lye E. et al., 2004. The role of interleukin 1 receptor-associated kinase-4 (IRAK-4) kinase activity in IRAK-4-mediated signaling. J Biol Chem. 279(39):40653-8.
4. Suzuki N et al., 2003. IL-1 receptor-associated kinase 4 is essential for IL-18-mediated NK and Th1 cell responses. J Immunol. 170(8):4031-5.

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Specifications

Human IRAK1 isoform 1 (pUNO1-hIRAK1a)

ORF size : 2139 bp
Subclone : AgeI - NheI

Human IRAK1-DN (pUNO1-hIRAK1-DN)

ORF size : 636 bp
Subclone : NcoI - NheI

Mouse IRAK1 isoform 4 (pUNO1-mIRAK1d)

Genbank : NM_008363.2 with silent variation in codon Phe708
ORF size : 2133 bp
Subclone : AgeI - EcoRI

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Contents pUNO1

  • 20 µg of lyophilized DNA
  • 2 x 1 ml blasticidin at 10 mg/ml

 

room temperature Product is shipped at room temperature.

store Lyophilized DNA should be stored at -20 ̊C.

stability Resuspended DNA should be stored at -20 ̊C and is stable up to 1 year.

Alert Blasticidin is a harmful compound. Refer to safety data sheet for handling instructions.

 

Store blasticidin at 4°C or -20°C for up to two years. Product is stable 2 weeks at 37°C.

Avoid repeated freeze-thaw cycles.

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Details

IRAK1-DN version

Dominant negative IRAK1 (aa1-211) is a truncated form of IRAK1 that contains the N-terminal death domain but lacks the protein-kinase domain. This IRAK1-DN was shown to inhibit MyD88-induced NF-κB activation, suggesting that IRAK1 and MyD88 interact through their death domains [1].

1. Burns K. et al., 1998. MyD88, an adapter protein involved in interleukin-1 signaling. J Biol Chem. 273(20):12203-9.

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Citations

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