Human IL-28b (IFN-λ3) Antibody

Mouse IgG1 (clone 18F4) - Hybridoma

ABOUT

Neutralizing monoclonal antibody against human interleukin 28B

Anti-hIL-28b-IgG (clone 18F4) is a monoclonal antibody (mAb) specific for human interleukin 28B (hIL-28B). It is produced in hybridomas and purified by affinity chromatography. This antibody has been selected for its ability to efficiently neutralize the biological activity of hIL-28B. IL-28B (also known as interferon lambda 3) is a member of the type III interferon (IFN lambda) cytokine family that exhibits several common features with type I IFNs: antiviral activity and antitumor activity [1, 2].

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Key features:

  • A potent and specific neutralizing mAb against hIL-28B
  • Provided azide-free
  • Each lot is functionally tested

 

References:

1. Donnelly RP. & Kotenko SV., 2010. Interferon-lambda: a new addition to an old family. J Interferon Cytokine Res. 30(8):555-64.
2. Li M. et al., 2009. Interferon-ls: the modulators of antivirus, antitumor, and immune responses. J. Leukoc. Biol., 86:23-32.

All products are for research use only, and not for human or veterinary use.

Neutralizing monoclonal antibody against human IL-28B
Neutralizing monoclonal antibody against human IL-28B

SPECIFICATIONS

Specifications

Target

IL-28B

Target species

Human

Species
Human
Isotype
mIgG1
kappa
Recommended isotype control
Mouse IgG1 Isotype Control Antibody
Clone
18F4
Tag
Tag-free
Source
Hybridoma
Purification
Affinity chromatography
Preservative
Azide-free
Purity
≥ 95 %
Appearance (form)
Lyophilized
Reconstitution buffer
Sterile water (not provided)
Endotoxin

Negative (tested using EndotoxDetect™ assay)

Tested applications

Neutralization assay

Quality control

Each lot is functionally tested and validated.

CONTENTS

Contents

  • Product: 
    Anti-hIL-28b-IgG
  • Cat code: 
    mabg-hil28b-3
  • Quantity: 
    3 x 100 µg

Shipping & Storage

  • Shipping method:  Room temperature

    • Storage:

    • -20°C
    Stability: -20°C for up to 1 year

    Caution:

    • Avoid repeated freeze-thaw cycles

Details

The IFN-λ family is essential to the antiviral response at epithelial barriers [1,2]. In humans, this family comprises four distinct proteins called IFN-λ1 (interleukin-29, IL-29), IFN-λ2 (IL-28A), IFN-λ3 (IL-28B), and the poorly secreted IFN-λ4. In mice, two functional orthologs (IL-28A and IL-28B) have been described. IFN-λs are produced when a viral infection is sensed by pattern recognition receptors. IFN-λs bind to a heterodimeric receptor formed by the assembly of IFNLR1 and IL10Rβ. This leads to the recruitment of the Janus kinases, JAK1 and TyK2, and phosphorylation of STAT1 and STAT2, which then dimerize and interact with IFN regulatory factor 9 (IRF9), forming the ISGF3 complex. ISGF3 binds to IFN-stimulated response elements (ISRE) in the promoters of IFN-stimulated genes (ISG) to regulate their expression.

 

1. Lazear HM. et al., 2015. Interferon-λ: Immune Functions at Barrier Surfaces and Beyond. Immunity. 43(1):15-28.
2. Lee S. & Baldridge MT., 2017. Interferon- Lambda: A Potent Regulator of Intestinal Viral Infections. Front Immunol. 8:749. 

DOCUMENTS

Documents

Anti-hIL-28b-IgG

Technical Data Sheet

Safety Data Sheet

View document

Validation Data Sheet

Certificate of analysis

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