Human IL-28B (IFN-λ3) Antibody

Mouse IgG1 (clone 18F4) - Recombinant

ABOUT

Recombinant anti-human IL-28B neutralizing monoclonal antibody

Anti-hIL-28b-mIgG1 is a monoclonal antibody (mAb) targeting human interleukin 28B (hIL-28B).

It was previously extracted from hybridoma cells (clone 18F4). It is now expressed and produced in Chinese hamster ovary (CHO) cells, ensuring reliability and lot-to-lot reproducibility. This antibody has been selected for its ability to efficiently neutralize the biological activity of hIL-28B.

IL-28B (also known as interferon lambda 3) is a member of the type III interferon (IFN lambda) cytokine family that exhibits several common features with type I IFNs: antiviral activity and antitumor activity [1, 2].

More details More details

This antibody can be used together with HEK-Blue™ IFN-λ Cells treated with recombinant human IL-28B for screening and neutralization assays to block type III interferon signaling.

 

Key features

  • Each lot is functionally tested and validated.
  • The complete sequence of the antibody construct has been verified.

  • The absence of endotoxins is determined by the EndotoxDetect™ assay.

     

The hybridoma-derived Anti-hIL-28b-IgG (mabg-hil28b-3) antibody has been replaced by Anti-hIL-28b-mIgG1 (mab9-hil28b), which is produced by recombinant technology and purified from CHO cells.

 

References:

1. Donnelly RP. & Kotenko SV., 2010. Interferon-lambda: a new addition to an old family. J Interferon Cytokine Res. 30(8):555-64.
2. Li M. et al., 2009. Interferon-ls: the modulators of antivirus, antitumor, and immune responses. J. Leukoc. Biol., 86:23-32.

All products are for research use only, and not for human or veterinary use.

Binding of Anti-hIL-28b mAb
Binding of Anti-hIL-28b mAb

SPECIFICATIONS

Specifications

Target

IL-28B, IFN-λ3

Target species

Human

Applications

Neutralization assay (tested)

Antibody applications
Neutralization assay
Species
Mouse
Isotype
mIgG1
kappa
Recommended isotype control
Mouse IgG1 Isotype Control Antibody
Clone
18F4
Tag
Tag-free
Source
CHO cells
Production details
Animal-free
Purification
Affinity chromatography
Formulation buffer

Sodium phosphate buffer with glycine, saccharose, and stabilizing agents

Preservative
Azide-free
Purity
≥ 95% (SDS-PAGE)
Appearance (form)
Lyophilized
Reconstitution buffer
Sterile water (not provided)
Endotoxin

Negative (tested using EndotoxDetect™ assay)

Tested applications

Neutralization

Quality control

Each lot is released only after meeting predefined biological activity specifications in a cell-based assay.

CONTENTS

Contents

  • Product: 
    Anti-hIL-28b-mIgG1
  • Cat code: 
    mab9-hil28b
  • Quantity: 
    100 µg

Shipping & Storage

  • Shipping method:  Room temperature

    • Storage:

    • -20°C
    Stability: -20°C for up to 1 year

    Caution:

    • Avoid repeated freeze-thaw cycles

Details

The IFN-λ family is essential to the antiviral response at epithelial barriers [1,2]. In humans, this family comprises four distinct proteins called IFN-λ1 (interleukin-29, IL-29), IFN-λ2 (IL-28A), IFN-λ3 (IL-28B), and the poorly secreted IFN-λ4. In mice, two functional orthologs (IL-28A and IL-28B) have been described. IFN-λs are produced when a viral infection is sensed by pattern recognition receptors. IFN-λs bind to a heterodimeric receptor formed by the assembly of IFNLR1 and IL10Rβ. This leads to the recruitment of the Janus kinases, JAK1 and TyK2, and phosphorylation of STAT1 and STAT2, which then dimerize and interact with IFN regulatory factor 9 (IRF9), forming the ISGF3 complex. ISGF3 binds to IFN-stimulated response elements (ISRE) in the promoters of IFN-stimulated genes (ISG) to regulate their expression.

 

1. Lazear HM. et al., 2015. Interferon-λ: Immune Functions at Barrier Surfaces and Beyond. Immunity. 43(1):15-28.
2. Lee S. & Baldridge MT., 2017. Interferon- Lambda: A Potent Regulator of Intestinal Viral Infections. Front Immunol. 8:749. 

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