Anti-hIL-28a-mIgG1
-
Cat.code:
mab9-hil28a
- Documents
ABOUT
Recombinant anti-human IL-28A neutralizing monoclonal antibody
Anti-hIL-28a-mIgG1 is a monoclonal antibody (mAb) targeting human interleukin 28A (hIL-28A).
It was previously extracted from hybridoma cells (clone 21C3). It is now expressed and produced in Chinese hamster ovary (CHO) cells, ensuring reliability and lot-to-lot reproducibility. This antibody has been selected for its ability to efficiently neutralize the biological activity of hIL-28A.
IL-28A (also known as interferon lambda 2, IFN-λ2, IFN-l2) is a member of the type III interferon (IFN lambda) cytokine family that exhibits several common features with type I IFNs: antiviral activity and antitumor activity [1, 2].
This antibody can be used together with HEK-Blue™ IFN-λ Cells treated with recombinant human IL-28A for screening and neutralization assays to block type III interferon signaling.
Key features
- Each lot is functionally tested and validated.
- The complete sequence of the antibody construct has been verified.
The absence of endotoxins is determined by the EndotoxDetect™ assay.
The hybridoma-derived Anti-hIL-28a-IgG (mabg-hil28a-3) antibody has been replaced by Anti-hIL-28a-mIgG1 (mab9-hil28a), which is produced by recombinant technology and purified from CHO cells.
References:
1. Donnelly RP. & Kotenko SV., 2010. Interferon-lambda: a new addition to an old family. J Interferon Cytokine Res. 30(8):555-64.
2. Li M. et al., 2009. Interferon-ls: the modulators of antivirus, antitumor, and immune responses. J. Leukoc. Biol., 86:23-32.
All products are for internal research use only, and not for human or veterinary use.
SPECIFICATIONS
Specifications
IL-28A, IFN-λ2
Human
No cross-reactivity with mouse IL-28A or mouse IL-28B.
Neutralization assay (tested)
Sodium phosphate buffer with glycine, saccharose, and stabilizing agents
Negative (tested using EndotoxDetect™ assay)
Neutralization
Each lot is functionally tested and validated.
Each lot is released only after meeting predefined biological activity specifications in a cell-based assay.
CONTENTS
Contents
-
Product:Anti-hIL-28a-mIgG1
-
Cat code:mab9-hil28a
-
Quantity:100 µg
Shipping & Storage
- Shipping method: Room temperature
- -20°C
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
Details
The IFN-λ family is essential to the antiviral response at epithelial barriers[1,2]. In humans, this family comprises four distinct proteins called IFN-λ1 (interleukin-29, IL-29), IFN-λ2 (IL-28A), IFN-λ3 (IL-28B), and the poorly secreted IFN-λ4. In mice, two functional orthologs (IL-28A and IL-28B) have been described. IFN-λs are produced when a viral infection is sensed by pattern recognition receptors. IFN-λs bind to a heterodimeric receptor formed by the assembly of IFN lambda receptor 1 (IFNLR1) and IL-10 receptor beta (IL10Rβ). This leads to the recruitment of the Janus kinases, JAK1 and TyK2, and phosphorylation of STAT1 and STAT2, which then dimerize and interact with IFN regulatory factor 9 (IRF9), forming the ISGF3 complex. ISGF3 binds to IFN-stimulated response elements (ISRE) in the promoters of IFN-stimulated genes (ISG) to regulate their expression.
1. Lazear HM. et al., 2015. Interferon-λ: Immune Functions at Barrier Surfaces and Beyond. Immunity. 43(1):15-28.
2. Lee S. & Baldridge MT., 2017. Interferon- Lambda: A Potent Regulator of Intestinal Viral Infections. Front Immunol. 8:749.
DOCUMENTS
Documents
Technical Data Sheet
Validation Data Sheet
Safety Data Sheet
Certificate of analysis
Need a CoA ?
