TLR-GFP fusion genes were generated by fusing the green fluorescent protein (GFP) gene to the C terminus of various human Toll-like receptor (TLR) genes (TLR1 to TLR6).
These TLR-GFP fusion proteins can be used to study the localization of the TLRs. Transfected cells can be analyzed for GFP expression by flow cytometry and Western-blotting using GFP antibodies.
The TLR-GFP fusion genes are cloned in the pUNO1 plasmid under the control of the strong and ubiquitous mammalian promoter EF1α/HTLV.
The pUNO1 plasmid is selectable with blasticidin in both E. coli and mammalian cells.