Product Unit size Cat. code Docs. Qty. Price


pUNO1 bearing the human NOD1 gene

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20 µg



pUNO1 bearing a dominant negative version of the human NOD1 gene

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20 µg



pUNO1 bearing the mouse NOD1 gene

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20 µg

  • About
  • Specifications
  • pUNO1 Contents
  • Details

Expression-ready ORF Clones

Alias : NLRC1, CARD4


Nucleotide-binding oligomerization domain containing 1
Caspase recruitment domain-containing protein 4


Available versions:

  • Wild type NOD1
  • Dominant negative NOD1 (see the "Details" tab)

Nucleotide-binding oligomerisation domain 1 (NOD1), also called caspase-recruitment domain (CARD)-4, is an intracellular pathogen-recognition molecule expressed ubiquitously in adult tissues. Upon bacterial invasion, NOD1 induces NF-κB activation pathways [1]. NOD1 recognizes a naturally occurring muropeptide of peptidoglycan (PGN) that presents a unique amino acid at its terminus called diaminopilemic acid (DAP) [2].
This amino acid is found mainly in the PGN of gram-negative bacteria designating NOD1 as a sensor of gram-negative bacteria. Recent studies show that signaling through NOD1 is able to activate NF-κB in cells infected with gram-negative bacteria that can bypass TLR activation [3].



1. Inohara N. et al., 1999. Nod1, an Apaf-1-like activator of caspase-9 and nuclear factor-kappaB. J Biol Chem. 274(21):14560-7.
2. Girardin SE. et al., 2003. Nod1 detects a unique muropeptide from gram-negative bacterial peptidoglycan. Science. 300(5625):1584-7.
3. Kim JG. et al., 2004. Nod1 is an essential signal transducer in intestinal epithelial cells infected with bacteria that avoid recognition by toll-like receptors. Infect Immun. 72(3):1487-95.

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Human NOD1 (pUNO1-hNOD1)

Genbank : NM_006092.2
ORF size : 2862 bp
Subclone : NcoI - EcoRI

Human NOD1-DN (pUNO1-hNOD1-DN)

ORF size : 2490 bp
Subclone : NcoI - EcoRI

Mouse NOD1 (pUNO1-mNOD1)

Genbank : NM_172729.3
ORF size : 2862 bp
Subclone : NcoI - EcoRI

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pUNO1 Contents

  • 20 µg of lyophilized DNA
  • 2 x 1 ml blasticidin at 10 mg/ml


room temperature Product is shipped at room temperature.

store Lyophilized DNA should be stored at -20°C. Upon receipt, store blasticidin at 4°C or -20°C.

stability Resuspended DNA should be stored at -20°C and is stable for up to 1 year.

Alert Blasticidin is a harmful compound. Refer to the safety data sheet for handling instructions.

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Dominant negative NOD1 was generated by a deletion of the centrally located nucleotide-binding site (NBS) domain (aa 127-518) [1].

1. Bertin JJ et al. 1999. Human CARD4 protein is a novel CED-4/Apaf-1 cell death family member that activates NF-κB. J Biol Chem. 274(19):12955-8

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