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NOD1

pUNO1-hNOD1 Unit size Cat. code Docs Qty Price
pUNO1 bearing the human NOD1 gene
20 µg
puno1-hnod1
+-
$384.00
pUNO1-hNOD1-DN Unit size Cat. code Docs Qty Price
pUNO1 bearing a dominant negative version of the human NOD1 gene
20 µg
puno1-hnod1-dn
+-
$406.00
pUNO1-mNOD1 Unit size Cat. code Docs Qty Price
pUNO1 bearing the mouse NOD1 gene
20 µg
puno1-mnod1
+-
$384.00

Expression-ready ORF Clones

Alias : NLRC1, CARD4

 

Nucleotide-binding oligomerization domain containing 1
Caspase recruitment domain-containing protein 4

 

Available versions:

  • Wild type NOD1
  • Dominant negative NOD1 (see the "Details" tab)

Nucleotide-binding oligomerisation domain 1 (NOD1), also called caspase-recruitment domain (CARD)-4, is an intracellular pathogen-recognition molecule expressed ubiquitously in adult tissues. Upon bacterial invasion, NOD1 induces NF-κB activation pathways [1]. NOD1 recognizes a naturally occurring muropeptide of peptidoglycan (PGN) that presents a unique amino acid at its terminus called diaminopilemic acid (DAP) [2].
This amino acid is found mainly in the PGN of gram-negative bacteria designating NOD1 as a sensor of gram-negative bacteria. Recent studies show that signaling through NOD1 is able to activate NF-κB in cells infected with gram-negative bacteria that can bypass TLR activation [3].

 

References:

1. Inohara N. et al., 1999. Nod1, an Apaf-1-like activator of caspase-9 and nuclear factor-kappaB. J Biol Chem. 274(21):14560-7.
2. Girardin SE. et al., 2003. Nod1 detects a unique muropeptide from gram-negative bacterial peptidoglycan. Science. 300(5625):1584-7.
3. Kim JG. et al., 2004. Nod1 is an essential signal transducer in intestinal epithelial cells infected with bacteria that avoid recognition by toll-like receptors. Infect Immun. 72(3):1487-95.

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Specifications

Human NOD1 (pUNO1-hNOD1)

Genbank : NM_006092.2
ORF size : 2862 bp
Subclone : NcoI - EcoRI

Human NOD1-DN (pUNO1-hNOD1-DN)

ORF size : 2490 bp
Subclone : NcoI - EcoRI

Mouse NOD1 (pUNO1-mNOD1)

Genbank : NM_172729.3
ORF size : 2862 bp
Subclone : NcoI - EcoRI

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Contents pUNO1

  • 20 µg of lyophilized DNA
  • 2 x 1 ml blasticidin at 10 mg/ml

 

room temperature Product is shipped at room temperature.

store Lyophilized DNA should be stored at -20 ̊C.

stability Resuspended DNA should be stored at -20 ̊C and is stable up to 1 year.

Alert Blasticidin is a harmful compound. Refer to safety data sheet for handling instructions.

 

Store blasticidin at 4°C or -20°C for up to two years. Product is stable 2 weeks at 37°C.

Avoid repeated freeze-thaw cycles.

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Details

NOD1-DN

Dominant negative NOD1 was generated by a deletion of the centrally located nucleotide-binding site (NBS) domain (aa 127-518) [1].

1. Bertin JJ et al. 1999. Human CARD4 protein is a novel CED-4/Apaf-1 cell death family member that activates NF-κB. J Biol Chem. 274(19):12955-8

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Citations

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