pNiFty2 plasmids are composed of either an ELAM proximal promoter, five NF-κB repeated transcription factor binding sites (TFBS) and a reporter gene : SEAP (Secreted alkaline phosphatase) or Luc (Luciferase), or an  ISG-56K or mouse interferon promoter and a  SEAP (Secreted alkaline phosphatase) reporter gene.

pNiFty2 plasmids are selectable with Zeocin™ in both E. coli and mammalian cells, and can be used to generate stable clones.

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