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Lucia®-tagged Fc-fusion protein expression plasmid - Human IgG2
Production of Lucia luciferase-tagged IgG2-Fc fusion proteins
pFUSEN-Lucia-hG2Fc is a cloning plasmid for the generation of a Lucia luciferase-tagged Fc-fusion protein expressing the Fc region (CH2 and CH3 domains) of the human IgG2 heavy chain and the hinge region fused to the N-terminus of a protein of interest.
Lucia is a secreted luciferase reporter protein with advantageous characteristics when associated with Fc-fusion proteins. It possesses superior carrier ability for excellent secretion of the chimeric protein. It provides a simple means to screen for recombinant clones and it minimally affects the activity of the protein of interest.
Human IgG2-Fc displays low affinity towards FcgRs and complement receptors. This Fc domain is more suitable for applications for which ADCC and CDC are not desirable.
Isotype: Human IgG2
Effector Activities: ADCC+/-, CDC +
Selectable with Zeocin™ in E. coli and mammalian cells.
For transient or stable transfection.
These products are covered by a Limited Use License (See Terms and Conditions).Back to the top
• 20 μg of lyophilized DNA
• 4 pouches of E. coli Fast-Media® Zeo (2 TB and 2 Agar)
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