pFUSEN-Lucia-hG2Fc
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
|---|---|---|---|---|---|---|
|
pFUSEN-Lucia-hG2Fc Lucia luciferase-tagged Fc-fusion protein expression plasmid - Human IgG2 |
Show product |
20 µg |
pfcn-lchg2
|
|
Production of Lucia luciferase-tagged IgG2-Fc fusion proteins
pFUSEN-Lucia-hG2Fc is a cloning plasmid for the generation of a Lucia luciferase-tagged Fc-fusion protein expressing the Fc region (CH2 and CH3 domains) of the human IgG2 heavy chain and the hinge region fused to the N-terminus of a protein of interest.
Lucia luciferase is a secreted reporter protein with advantageous characteristics when associated with Fc-fusion proteins.
It possesses superior carrier ability for excellent secretion of the chimeric protein. It provides a simple means to screen for recombinant clones and it minimally affects the activity of the protein of interest.
Human IgG2-Fc displays low affinity towards FcgRs and complement receptors. This Fc domain is more suitable for applications for which ADCC and CDC are not desirable.
Back to the topSpecifications
Isotype: Human IgG2
Effector Activities: ADCC+/-, CDC +
Selectable with Zeocin™ in E. coli and mammalian cells.
For transient or stable transfection.
These products are covered by a Limited Use License (See Terms and Conditions).
Back to the toppFUSEN Contents
- 20 µg of lyophilized DNA
- 1 ml of Zeocin™ (100 mg/ml)
Product is shipped at room temperature
