MycoStrip™ - Mycoplasma Detection Kit
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Mycoplasma Detection Kit
Easy and fast detection of mycoplasma contamination in cell cultures
With over 40 years of experience in developing mycoplasma solutions for the scientific community, InvivoGen's MycoStrip™ offers a new method for the detection of mycoplasma in cell culture, allowing you to combat the contamination.
MycoStrip™ is a simple test that requires no special lab equipment, and provides clear results, with no false positives. Furthermore, with results in less than an hour, MycoStrip™ allows you to swiftly start treating the contaminated cell culture.
Detection of cell culture contaminating mycoplasma by MycoStrip™ is based on isothermal PCR. Simply prepare your sample and add our proprietary Reaction Mix to target and amplify the 16S rRNA gene for the most commonly found mycoplasma species in cell culture. Results are clearly visualized on an immunochromatographic strip within 5 minutes.
Key features of MycoStrip™
Easy to perform, and unlike most other mycoplasma detection assays, no special lab equipment is required.
Performed in 1 hour, with less than 15 minutes of hands-on time.
Fast and easy-to-interpret results that appear within 2-5 minutes, as 1 or 2 bands on the strip.
- One band - negative for mycoplasma
- Two bands - positive for mycoplasma
MycoStrip™ has been specifically designed to detect the Mycoplasma and Acholeplasma species that most commonly contaminate cell culture. These include the six species that account for 95% of all contaminations: M. orale, M. hyorhinis, M. arginini, M. fermentans, M. hominis, and A. laidlawii. Importantly, because the MycoStrip™ reaction mix detects unique regions of DNA there is no cross‑reactivity with other bacterial, fungal or mammalian DNA.
MycoStrip™ is able to detect as low as 10-102 CFU/ml and thus, can be used to detect mycoplasma contamination before it significantly affects experimental results, which typically occurs at ~107 CFU/ml .
1. Drexler, H.G. & Uphoff, C.C. 2002. Mycoplasma contamination of cell cultures: Incidence, sources, effects, detection, elimination, prevention. Cytotechnology 39, 75-90.
There are two distinct results using MycoStrip™ One band (‘C’ band only) indicates that mycoplasma was not detected in the processed sample. Two bands (‘C’ and ‘T’ band) indicate that the processed sample is contaminated by mycoplasma. The migration control band (‘C’) will appear within 1-2 minutes. If after 5 minutes there is no test band (‘T’), the test is considered negative.
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• Reaction Mix
• Reaction Buffer
• Migration Buffer
• Positive Control
• Detection strips
MycoStrip™ is shipped at room temperature.
Store all components (including the detection strips) at -20°C.
If properly stored, all components are stable for 12 months.
Mycoplasma is a major problem in cell culture, with various sources of contamination such as lab operators, dirty water baths/incubators, and biological reagents. Due to their small size, mycoplasma is undetectable by visual inspection, they pass through standard filtration, and are resistant to a large number of antibiotics. There are many reasons to regularly test your cell culture for mycoplasma and these include:
- Loss of precious samples
- Serious impact on data reliability and reproducibility
- Testing is required by most journals for publication
- Loss of time and money
1. What if I am positive for mycoplasma?
InvivoGen has the solution! Your culture is easily treatable with our comprehensive anti-mycoplasma reagents. Simply treat your culture with Plasmocin™ or Plasmocure™ and upon completion of the treatment (~2 weeks), re-test using MycoStrip™.
Note: For more information check out our Practical Guide to Cell culture contamination.
2. I have collected some cells with the supernatant that is to be tested, will this interfere with the assay?
There is no need to worry, the presence of mammalian cell DNA will not affect the detection assay.
3. During sample preparation, is there an advantage to heating the sample to 95°C?
There is no increase in sensitivity however this step will kill the mycoplasma avoiding the handling of live mycoplasma.
4. Is it necessary to include a positive and negative control every time when using MycoStrip™?
It is important to include positive and negative controls on a regular basis to monitor the reliability of your results and in case of troubleshooting.
5. At what stage can I freeze my sample for later analysis?
Samples can be stored as followed:
- Cell culture supernatant: before starting the “preparation of samples” protocol, you can store the supernatant at -20°C until required. Before use, completely thaw the sample at 37°C and mix well by vortexing to ensure all salts are dissolved.
- Prepared sample: After performing steps 1 – 4 of the “preparation of samples” protocol, your sample can be store at -20°C until required.
- Processed sample: After performing steps 1 – 4 of the “detection assay” protocol, the processed samples can be stored at -20°C for up to 3 months.
6. I have a faint test band for one of my samples and am unsure if this should be considered as positive for mycoplasma contamination. What would you recommend?
If you observe a faint band for one of your samples, we consider this to be a positive result for mycoplasma contamination. We recommend confirming this result using one of the following tips:
- Concentrate your sample: begin with a larger volume of supernatant and/or repeat steps 2-3 in the “preparation of samples” protocol. Re‑test the concentrated sample using MycoStrip™.
- Continue to grow the culture for an additional 48 hours before re-testing using MycoStrip™.
- Use sterile water (nuclease-free) to resuspend the sample instead of PBS (to increase the sensitivity of the test).
7. I do not have a heat block in the lab, what would you recommend to use instead?
If you do not have a heat block, you can use a thermocycler or water bath instead. Please note that the use of a water bath can introduce new sources of contamination.
8. I have left my prepared sample for over an hour on the heat block and wonder if I can still use this sample?
Exceeding the 40-minute incubation at 65°C significantly impacts the results of the assay and therefore, we do not recommend to use this sample.