Human IL-1α Antibody

Mouse IgG1 (clone 7D4) - Recombinant

ABOUT

Recombinant anti-human IL-1α neutralizing monoclonal antibody

Anti-hIL-1α-mIgG1 is a monoclonal antibody (mAb) targeting human interleukin 1 alpha (hIL-1α).

It was previously extracted from hybridoma cells (clone A5-7-D4 ssD1). It is now expressed and produced in Chinese hamster ovary (CHO) cells, ensuring reliability and lot-to-lot reproducibility. This antibody has been selected for its ability to efficiently neutralize the biological activity of hIL-1α.

IL-1α is a pro-inflammatory cytokine that plays a central role in the regulation of immune responses and inflammation [1]. 

More details More details

This antibody can be used together with HEK-Blue™ IL-1β Cells treated with recombinant human IL-1α for screening and neutralization assays to block type III interferon signaling.

 

Key features

  • Each lot is functionally tested and validated.
  • The complete sequence of the antibody construct has been verified.

  • The absence of endotoxins is determined by the EndotoxDetect™ assay.

     

The hybridoma-derived Anti-hIL-1α-IgG (mabg-hil1a-3) antibody has been replaced by Anti-hIL-1α-mIgG1 (mab9-hil1a), which is produced by recombinant technology and purified from CHO cells.

 

Reference:

1. Dinarello C., 2018. Overview of the IL-1 family in innate inflammation and acquired immunity. Immunol Rev. 281(1): 8–27. 

All InvivoGen products are for internal research use only, and not for human or veterinary use.

Binding of Anti-hIL-1α mAb
Binding of Anti-hIL-1α mAb

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SPECIFICATIONS

Specifications

Target

IL-1α

Target species

Human

Crossreactivity

No cross-reactivity with human IL-1β, mouse IL-1α, or mouse IL-1β

Antibody applications
Neutralization assay
Species
Mouse
Isotype
mIgG1
kappa
Recommended isotype control
Recombinant Mouse IgG1 Isotype Control Antibody
Clone
A5-7-D4 ssD1
Tag
Tag-free
Source
CHO cells
Production details
Animal-free
Purification
Affinity chromatography
Formulation buffer

Sodium phosphate buffer with glycine, saccharose, and stabilizing agents

Preservative
Azide-free
Purity
≥ 95% (SDS-PAGE)
Appearance (form)
Lyophilized
Reconstitution buffer
Endotoxin-free water (not provided)
Endotoxin

Negative (tested using EndotoxDetect™ assay)

Tested applications

Neutralization assay

Quality control

Each lot is functionally tested and validated.

CONTENTS

Contents

  • Product: 
    Anti-hIL-1α-mIgG1
  • Cat code: 
    mab9-hil1a
  • Quantity: 
    100 µg

Shipping & Storage

  • Shipping method:  Room temperature

    • Storage:

    • -20°C
    Stability: -20°C for up to 1 year

    Caution:

    • Avoid repeated freeze-thaw cycles

Details

Interleukin-1 alpha (IL-1α) is a secreted pro-inflammatory cytokine produced primarily by activated macrophages [1]. IL-1α is synthesized as a pro‑cytokine, which is enzymatically cleaved into the mature secreted form by calpain, a calcium-dependent protease [2]. In contrast to other cytokines, both the precursor and mature forms of IL-1α are biologically active.

IL-1α plays a central role in the regulation of immune responses and inflammation. IL-1α binds to the interleukin-1 receptor, leading to the activation of NF-κB signaling and the JNK and p38 mitogen-activated protein kinase pathways, which induce the expression of inflammatory cytokines and chemokines, such as IL-6 and IL-8 [3].

 

1. Dinarello C., 2018. Overview of the IL-1 family in innate inflammation and acquired immunity. Immunol Rev. 281(1): 8-27.
2. Sims J. & Smith D., 2010. The IL-1 family: regulators of immunity. Nat Rev Immunol. 10(2):89-102.
3. Weber A. et al., 2010. Interleukin-1 (IL-1) pathway. Sci Signal. 3(105):cm1.

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