Anti-hIL-1α Neutralizing mAb
|Anti-hIL-1α-IgG||Unit size||Cat. code||Docs||Qty||Price|
Human IL-1α Neutralizing antibody (clone 7D4) - Monoclonal Human IgG1
3 x 100 µg
Neutralizing monoclonal antibody against human interleukin 1 alpha
Anti-hIL-1α-IgG (clone 7D4) is a monoclonal antibody (mAb) specific for human interleukin 1 alpha (hIL-1α). It is produced in hybridomas and purified by affinity chromatography. This antibody has been selected for its ability to efficiently neutralize the biological activity of hIL-1α.
IL-1α is a pro-inflammatory cytokine that plays a central role in the regulation of immune responses and inflammation .
- A potent and specific neutralizing mAb against hIL-1α
- Provided azide-free
- No cross-reactivity with human IL-1β, murine IL-1α, or murine IL-1β
- Each lot is functionally tested
1. Dinarello C., 2018. Overview of the IL-1 family in innate inflammation and acquired immunity. Immunol Rev. 281(1): 8–27.Back to the top
Target: Natural and recombinant human interleukin-1 alpha (IL-1α)
Specificity: No cross-reactivity with human IL-1β, murine IL-1α, or murine IL-1β
Isotype: Mouse IgG1, kappa
Control: Mouse IgG1 Control
Immunogen: Human IL-1α protein expressed in Swiss mice following DNA immunization
Formulation: 0.2 µm filtered solution in a sodium phosphate buffer with glycine, saccharose, and stabilizing agents
Tested applications: Blocking & Neutralisation
- This product has been validated for neutralization.
- The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK‑Blue™ TLR4 cells.
- 3x 100 µg purified anti-hIL-1α-IgG provided lyophilized
The product is shipped at room temperature.
Upon receipt, store lyophilized antibody at -20 °C.
Reconstituted antibody is stable for 1 month at 4 °C and for 1 year at -20 °C.
Avoid repeated freeze-thaw cycles.Back to the top
Interleukin-1 alpha (IL-1α) is a secreted pro-inflammatory cytokine produced primarily by activated macrophages . IL-1α is synthesized as a pro‑cytokine, which is enzymatically cleaved into the mature secreted form by calpain, a calcium-dependent protease . In contrast to other cytokines, both the precursor and mature forms of IL-1α are biologically active.
IL-1α plays a central role in the regulation of immune responses and inflammation. IL-1α binds to the interleukin-1 receptor, leading to the activation of NF-κB signaling and the JNK and p38 mitogen-activated protein kinase pathways, which induce the expression of inflammatory cytokines and chemokines, such as IL-6 and IL-8 .
1. Dinarello C., 2018. Overview of the IL-1 family in innate inflammation and acquired immunity. Immunol Rev. 281(1): 8-27.
2. Sims J. & Smith D., 2010. The IL-1 family: regulators of immunity. Nat Rev Immunol. 10(2):89-102.
3. Weber A. et al., 2010. Interleukin-1 (IL-1) pathway. Sci Signal. 3(105):cm1.