pINFUSE-hIgG4-Fc

Fc-fusion protein expression plasmid - Human IgG4 with introns

ABOUT

INFUSE-hIgG4-Fc1 is a cloning plasmid for the generation of a Fc-fusion protein expressing the Fc region (CH2 and CH3 domains) of the human IgG4 heavy chain and the hinge region. The hinge serves as a flexible spacer between the two parts of the Fc-fusion protein, allowing each part of the molecule to function independently. A short intron is present between each region (one intron between the hinge and CH2 and one intron between CH2 and CH3). The presence of introns is known to enhance the level of gene expression as splicing is known to promote rapid and efficient mRNA export.

pINFUSE-hIgG4-Fc2 additionally features the IL2 signal sequence for the generation of Fc-Fusion proteins derived from proteins that are not naturally secreted

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Applications

Transient or stable transfection in vitro

Species
Human
Tag
Fc, N-ter
Plasmid backbone
pFUSE
Antibiotic resistance
Zeocin®
Purification
Ion-exchange chromatography
Appearance (form)
Translucent film
Appearance (color)
Colorless
Reconstitution buffer
Sterile water (not provided)
Quality control

Plasmid construct has been confirmed by restriction analysis and sequencing.

CONTENTS

Contents

  • Product: 
    pINFUSE-hIgG4-Fc1
  • Cat code: 
    pfc1-hgin40
  • Quantity: 
    20 µg
Includes:

1 ml of Zeocin™ (100 mg/ml)

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C
    Stability: Reconstituted product is stable for at least 1 year at -20 °C.

DOCUMENTS

Documents

pINFUSE-hIgG4-Fc1

Technical Data Sheet

Safety Data Sheet

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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