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ASC-GFP

pSELECT-hASC-GFP Unit size Cat. code Docs Qty Price
hASC::GFP fusion gene
20 µg
psetz-hascgfp
+-
$448.00
pSELECT-mASC-GFP Unit size Cat. code Docs Qty Price
mASC::GFP fusion gene
20 µg
psetz-mascgfp
+-
$448.00

A mammalian expression plasmid encoding an ASC::GFP fusion protein 

 

pSELECT-ASC-GFP are mammalian expression vectors designed for the expression of the human or murine ASC genes fused to the green fluorescent protein (GFP). These plasmids are selectable in bacteria and mammalian cells with Zeocin™.

pSELECT-ASC-GFP plasmids contain two expression cassettes. The first delivers strong and constitutive expression of hASC::GFP or mASC::GFP. The second drives the zeocin-resistance gene (Sh ble) that allows the selection of stable transfectants (and selection in bacteria). Both cassettes are terminated with a strong polyadenylation signal that separates the two expression cassettes thus preventing any transcription interference.

In transfected cells equipped with inflammasome components, ASC::GFP expression enables the visual monitoring of ASC specks, a hallmark of inflammasome activation .

The same plasmid is available with the GFP gene alone as a control (pSELECT-CGFP-zeo).

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Specifications

Selection: Zeocin™

Gene: ASC::GFP fusion gene

Promoter: hEF1-HTLV

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Contents pUNO1

  • 20 µg of lyophilized DNA
  • 2 x 1 ml blasticidin at 10 mg/ml

 

room temperature Product is shipped at room temperature.

store Lyophilized DNA should be stored at -20 ̊C.

stability Resuspended DNA should be stored at -20 ̊C and is stable up to 1 year.

Alert Blasticidin is a harmful compound. Refer to safety data sheet for handling instructions.

 

Store blasticidin at 4°C or -20°C for up to two years. Product is stable 2 weeks at 37°C.

Avoid repeated freeze-thaw cycles.

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