ASC-GFP
| pSELECT-hASC-GFP | Unit size | Cat. code | Docs | Qty | Price |
|---|---|---|---|---|---|
hASC::GFP fusion gene |
20 µg |
psetz-hascgfp |
| pSELECT-mASC-GFP | Unit size | Cat. code | Docs | Qty | Price |
|---|---|---|---|---|---|
mASC::GFP fusion gene |
20 µg |
psetz-mascgfp |
A mammalian expression plasmid encoding an ASC::GFP fusion protein
pSELECT-ASC-GFP are mammalian expression vectors designed for the expression of the human or murine ASC genes fused to the green fluorescent protein (GFP). These plasmids are selectable in bacteria and mammalian cells with Zeocin™.
pSELECT-ASC-GFP plasmids contain two expression cassettes. The first delivers strong and constitutive expression of hASC::GFP or mASC::GFP. The second drives the zeocin-resistance gene (Sh ble) that allows the selection of stable transfectants (and selection in bacteria). Both cassettes are terminated with a strong polyadenylation signal that separates the two expression cassettes thus preventing any transcription interference.
In transfected cells equipped with inflammasome components, ASC::GFP expression enables the visual monitoring of ASC specks, a hallmark of inflammasome activation .
The same plasmid is available with the GFP gene alone as a control (pSELECT-CGFP-zeo).
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- 20 µg of lyophilized DNA
- 2 x 1 ml blasticidin at 10 mg/ml
Product is shipped at room temperature.
Lyophilized DNA should be stored at -20 ̊C.
Resuspended DNA should be stored at -20 ̊C and is stable up to 1 year.
Blasticidin is a harmful compound. Refer to safety data sheet for handling instructions.
Store blasticidin at 4°C or -20°C for up to two years. Product is stable 2 weeks at 37°C.
Avoid repeated freeze-thaw cycles.
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