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Murine IgG1 Isotype Controls

Anti-β-Gal-mIgG1 Unit size Cat. code Docs Qty Price
Isotype control - Murine IgG1 antibody (clone T9C6). For in vitro use.
200 µg
bgal-mab9-02
+-
$208.00
Anti-β-Gal-mIgG1 InvivoFit™ Unit size Cat. code Docs Qty Price
Isotype control - Murine IgG1 antibody (clone T9C6). For in vivo use.
1 mg
10 mg
bgal-mab9-1
+-
$322.00

Recombinant mouse IgG1 isotype control mAb against E. coli β-galactosidase

InvivoGen’s Anti-β-Gal-mIgG1 antibody
InvivoGen’s Anti-β-Gal-mIgG1 antibody

InvivoGen provides a recombinant anti-β-Gal-mIgG1 monoclonal antibody (mAb)  that was previously extracted from hybridoma. It is now expressed and produced in chinese hamster ovary (CHO) cells, ensuring reliability and lot-to-lot reproducibility. Thereby, common hybridoma-related drawbacks such as the generation of non-relevant mAbs containing aberrant light chains are avoided. This control antibody targets E. coli's β-galactosidase (β-Gal), an antigen that is not present in mice. In addition, it displays low ADCC (antibody-dependent cell-mediated cytotoxicity) and no CDC (complement-dependent cytotoxicity) effector functions [1].

The sequence of Anti-β-Gal-mIgG1 is 100% murine (constant and variable regions), as the original clone (clone T9C6) was raised in mice using a proprietary method. This feature allows for reduced immunogenicity and risks of fatal hypersensitivity reactions upon repeated mAb injections into mice [2]. 

 

InvivoGen provides this antibody in two grades:

  • In vitro use: Anti-β-Gal-mIgG1
  • In vivo use:  Anti-β-Gal-mIgG1 InvivoFit™


All InvivoFit™ products are handled in a clean room, filter-sterilized, and tested for bacterial contaminants. Additionally, this grade guarantees low levels of endotoxins (<1 EU/ml). The buffer formulation is specifically adapted for in vivo studies.

 

Key features:

  • Targets E. coli β-galactosidase antigen 
  • Sequence is 100% murine
  • Murine IgG1 isotype (constant region)
  • Free from non-relevant mAbs found in hybridoma-based productions
  • Produced in animal-free facilities and defined media
  • Low aggregation < 5%
  • InvivoFit™ grade is available

 

References:

1. Bradbury, A. et al., 2018. When monoclonal antibodies are not monospecific: Hybridomas frequently express additional functional variable regions. mAbs, 10(4), 539–546.
2. Yamazaki T. et al., 2005. Blockade of B7-H1 on macrophages suppresses CD4+ T cell proliferation by augmenting IFN-gamma-induced nitric oxide production. J Immunol. 175(3):1586-92.

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Specifications

Target: E. coli β-galactosidase (β-Gal)

Specificity: no cross-reaction with murine proteins

Clone: T9C6

Source: CHO cells

Isotype: Murine IgG1, kappa

Formulation of Anti-β-Gal-mIgG1: Lyophilized from 0.2 µm filtered solution in a sodium phosphate buffer with glycine, saccharose, and stabilizing agents

Formulation of Anti-β-Gal-mIgG1 InvivoFit™: Lyophilized from 0.2 µm filtered solution in a sodium phosphate buffer with 5% saccharose

Application: Negative control for murine IgG1 antibodies

Quality control:

  • The absence of binding was confirmed by flow cytometry.
  • The complete sequence of this antibody has been verified.
  • The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK‑Blue™ TLR4 cells.
  • The endotoxin level in Anti-β-Gal-mIgG1 InvivoFit™ is <1 EU/mg (determined by a LAL assay).
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Contents

Please note: each mAb is sold separately.

Anti-β-Gal-mIgG1

  • bgal-mab9-02​: 200 µg, lyophilized

Anti-β-Gal-mIgG1 InvivoFit™

  • bgal-mab9-1​: 1 mg, lyophilized
  • bgal-mab9-10​: 10 mg, lyophilized

 

room temperature The product is shipped at room temperature.

store Store lyophilized antibody at -20 °C.

stability Lyophilized product is stable for at least 1 year.

Alert Avoid repeated freeze-thaw cycles.

 

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InvivoFit™

InvivoFit™ is a high-quality standard specifically adapted for in vivo studies. InvivoFit™ products are filter-sterilized (0.2 µm) and filled under strict aseptic conditions in a clean room. The level of bacterial contaminants (endotoxins and lipoproteins) in each lot is verified using a LAL assay and a TLR2 and TLR4 reporter assay.

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