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PKR

pUNO1-hPKR Unit size Cat. code Docs Qty Price
pUNO1 bearing the human PKR gene
20 µg
puno1-hpkr
+-
$384.00
pUNO1-hPKR-DN Unit size Cat. code Docs Qty Price
pUNO1 bearing a dominant negative version of the human PKR gene
20 µg
puno1-hpkr-dn
+-
$406.00
pUNO1-mPKR Unit size Cat. code Docs Qty Price
pUNO1 bearing the mouse PKR gene
20 µg
puno1-mpkr
+-
$384.00

Expression-ready ORF Clones

Alias : PRKR, EIF2AK2, EIF2AK1

 

Protein Kinase, Interferon-inducible double stranded RNA dependent.
Eukaryotic translation initiation factor 2-alpha kinase 2.

 

Available versions:

  • Wild type PKR
  • Dominant negative PKR (see the "Details" tab)

Protein kinase RNA-regulated (PKR), an ubiquitous serine/threonine kinase that is induced by interferons, is a critical signal transducer implicated in different stress-induced signaling pathways including dsRNA-induced TLR3 signaling [1] and LPS-induced TLR4 signaling [2,3]. Upon stress stimulation, PKR is activated by autophosphorylation and subsequently phosphorylates the alpha-subunit of eukaryotic initiation factor 2 (eIF-2a) thereby inducing apoptosis. PKR mediates the activation of important transcription factors, such as NF-kB, and MAP kinases, such as p382, by interacting with the TAK1 complex [4].
 

1. Jiang Z. et al., 2003. Poly(I-C)-induced Toll-like receptor 3 (TLR3)-mediated activation of NFkappa B and MAP kinase is through an interleukin-1 receptor-associated kinase (IRAK)-independent pathway employing the signaling components TLR3-TRAF6-TAK1-TAB2-PKR. J Biol Chem. 278(19):16713-9. 
2. Goh KC. et al., 2000. The protein kinase PKR is required for p38 MAPK activation and the innate immune response to bacterial endotoxin. EMBO J. 19(16):4292-7.
3. Hsu LC. et al., 2004. The protein kinase PKR is required for macrophage apoptosis after activation of Toll-like receptor 4. Nature. 2004 Mar 18;428(6980):341-5.

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Specifications

Human PKR (pUNO1-hPKR)

Genbank : NM_002759.3
ORF size : 1656 bp
Subclone : AgeI - NheI

Human PKR-DN (pUNO1-hPKR-DN)

ORF size : 1638 bp
Subclone : AgeI - NheI

Mouse PKR (pUNO1-mPKR)

ORF size : 1548 bp
Subclone : NcoI - NheI

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Contents

- 20 µg of lyophilized DNA.
- 4 pouches of E. coli Fast-Media® Blas (2 TB and 2 Agar)
- 1 ml blasticidin at 10 mg/ml

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Details

PKR-DN

Dominant negative PKR lacks six amino acids (aa361-366) between catalytic domains IV and V which render hPKR-DN unable to autophosphorylate or activate substrate proteins. Expression of hPKR-DN in NIH 3T3 cells resulted in a malignant tranformation, suggesting that PKR may function as a suppressor of cell proliferation and tumorigenesis [1].

1. Koromilas AE et al. 1992. Malignant transformation by a mutant of the IFN-inducible dsRNA-dependent protein kinase. Science 257(5077):1685-9.

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