HMGB1-Lucia
| pSELECT-hHMGB1-Lucia | Unit size | Cat. code | Docs | Qty | Price |
|---|---|---|---|---|---|
hHMGB1::Lucia fusion gene |
20 µg |
psetz-hhmgbluc |
| pSELECT-mHMGB1-Lucia | Unit size | Cat. code | Docs | Qty | Price |
|---|---|---|---|---|---|
mHMGB1::Lucia fusion gene |
20 µg |
psetz-mhmgbluc |
Expression-ready ORF Clones
pSELECT-HMGB1-Lucia are mammalian vectors designed for expression of the human or murine HMGB1::Lucia fusion genes. These plasmids are selectable in bacteria and mammalian cells with Zeocin™.
pSELECT-HMGB1::Lucia plasmids contain two expression cassettes.
- The first one delivers strong and constitutive expression of HMGB1::Lucia.
- The second one drives the zeocin-resistance gene (Sh ble) that allows the selection of stable transfectants (and selection in bacteria).
Each cassette terminates with a strong polyadenylation signal (polyA) thus preventing any transcription interference. In order to obtain enough copies for HMGB1::Lucia expression in mammalian cells, we recommend to use this plasmid for viral gene transfer.
Expression of HMGB1::Lucia in cells equipped for inflammasome mediated-pyroptosis and/or necroptosis allows monitoring of these forms of necrotic cell death.
Back to the topSpecifications
Selection: Zeocin™
Gene: HMGB1::Lucia fusion gene
Promoter: hEF1-HTLV
Back to the topContents
- 20 µg of lyophilized DNA
- 1 ml of Zeocin™ (100 mg/ml)
Product is shipped at room temperature