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HMGB1-Lucia

Product Unit size Cat. code Docs. Qty. Price

pSELECT-hHMGB1-Lucia

hHMGB1::Lucia fusion gene

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20 µg

psetz-hhmgbluc
+-
$554

pSELECT-mHMGB1-Lucia

mHMGB1::Lucia fusion gene

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20 µg

psetz-mhmgbluc
+-
$554
  • About
  • Specifications
  • Contents

Expression-ready ORF Clones

 

pSELECT-HMGB1-Lucia are mammalian vectors designed for expression of the human or murine HMGB1::Lucia fusion genes. These plasmids are selectable in bacteria and mammalian cells with Zeocin™.

pSELECT-HMGB1::Lucia plasmids contain two expression cassettes.

  • The first one delivers strong and constitutive expression of HMGB1::Lucia.
  • The second one drives the zeocin-resistance gene (Sh ble) that allows the selection of stable transfectants (and selection in bacteria).

Each cassette terminates with a strong polyadenylation signal (polyA) thus preventing any transcription interference. In order to obtain enough copies for HMGB1::Lucia expression in mammalian cells, we recommend to use this plasmid for viral gene transfer.

Expression of HMGB1::Lucia in cells equipped for inflammasome mediated-pyroptosis and/or necroptosis allows monitoring of these forms of necrotic cell death.

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Specifications

Selection: Zeocin™

Gene: HMGB1::Lucia fusion gene

Promoter: hEF1-HTLV

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Contents

  • 20 µg of lyophilized DNA
  • 1 ml of Zeocin™ (100 mg/ml)

room temperature Product is shipped at room temperature

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