Murine Anti-mCD3 mAb
Anti-mCD3-mIgG2a InvivoFit™ | Unit size | Cat. code | Docs | Qty | Price |
---|---|---|---|---|---|
145-2C11-derived mouse monoclonal antibody against murine CD3 |
1 mg 10 mg |
mcd3-mab10-1 |
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Recombinant mouse mAb against murine CD3ε for in vivo use
Anti-mCD3-mIgG2a InvivoFit™ is an anti-mCD3 monoclonal antibody featuring the variable region of the previously described 145-2C11 clone [1] and a murinized IgG2a constant region.
InvivoGen’s engineered Anti-mCD3-mIgG2a InvivoFit™ antibody
CD3 is a multimeric protein complex consisting of four polypeptides (CD3ε, CD3γ, CD3δ, and CD3ζ) that assemble as three dimers (εγ, εδ, and ζζ) [2]. The CD3 complex is a marker of T cells [2]. Upon antigen recognition, the TCR/CD3 complex on T cells triggers downstream intracellular signaling and participates in T cell activation [3].
The anti-mCD3ε 145-2C11 mAb is commonly used for cytometry detection and in vivo depletion of the T cell population [1]. Using recombinant technology, the original 145-2C11 Armenian Hamster IgG1 constant region has been replaced with a murine IgG2a format which mediates potent cytotoxic functions [4]. This murinization also allows reduced immunogenicity of the administered mAb and risks for fatal hypersensitivity reactions [5-7].
Key features:
- Derives from the 145-2C11 clone, Armenian Hamster IgG1
- Features the mIgG2a isotype (constant region)
- Guaranteed sterile, endotoxin level < 1 EU/mg
- Suitable for parental delivery in mice (azide-free)
- Low aggregation < 5%
- Produced in animal-free facilities and defined media
Anti-mCD3-mIgG2a InvivoFit™ is produced in Chinese hamster ovary (CHO) cells, purified by affinity chromatography with protein A, and provided in an InvivoFit™ grade, a high-quality standard specifically adapted to in vivo studies. The specific binding of this mAb to mCD3 has been confirmed by FACS and ELISA (see Figures).
References:
1. Leo O., et al., 1986. Identification of a monoclonal antibody specific for a murine T3 polypeptide. PNAS. 84(5):1374-1378.
2. Chetty R. & Gatter K., 1994. CD3: structure, function, and role of immunostaining in clinical practice. J. Pathol. 173(4):303-307.
3. Smith-Garvin J.E. et al., 2009. T Cell Activation. Ann. Rev. Immunol. 27:591-619.
4. Nimmerjahn F. & Ravetch J.V., 2005. Divergent immunoglobulin g subclass activity through selective Fc receptor binding. Science. 310(5753):1510-2.
5. Mall C. et al., 2016. Repeated PD-1/PD-L1 monoclonal antibody administration induces fatal xenogenic hypersensitivity reactions in a murine model of breast cancer. Onco Immunol. 5(2):e1075114.
6. Murphy, J.T. et al., 2014. Anaphylaxis caused by repetitive doses of a GITR agonist monoclonal antibody in mice. Blood. 123(14):2172-2180.
7. Belmar N.A. et al., 2017. Murinization and H chain isotype matching of Anti-GITR antibody DTA-1 reduces immunogenicity-mediated anaphylaxis in C57BL/6 mice. J Immunol. 198:4502-4512.
Specifications
Specificity: Targets cells expressing murine CD3ε
Formulation: Lyophilized from 0.2 μm filtered solution in 150 mM sodium chloride, 20 mM sodium phosphate buffer with 5% saccharose
Clonality: Monoclonal antibody
Isotype: Murine IgG2a, kappa
Control: mIgG2a InvivoFit™ isotype control
Source: CHO cells
Purity: Purified by affinity chromatography with protein A
Applications: Flow cytometry; in vivo depletion; ELISA
Quality control:
- Binding of Anti-mCD3-mIgG2a InvivoFit™ to mCD3ε has been confirmed using Flow cytometry
- Antibody binding has been validated by ELISA using a coated mouse CD3 protein
- The complete sequence of the antibody construct has been verified
- < 5% aggregates (confirmed by size exclusion chromatography)
- Guaranteed sterile and <1 EU/mg (determined by the LAL assay)
Contents
Anti-mCD3-mIgG2a InvivoFit™ is provided sterile, endotoxin-free, azide-free, and lyophilized.
This product is available in two pack sizes:
- mcd3-mab10-1: 1 mg
- mcd3-mab10-10: 10 mg
The product is shipped at room temperature.
Store lyophilized antibody at -20 °C.
Lyophilized product is stable for at least 1 year
Avoid repeated freeze-thaw cycles.
Details
CD3 Background:
The cluster of differentiation 3 (CD3, formerly named T3) is a multimeric protein complex consisting of four polypeptides (CD3ε, CD3γ, CD3δ, and CD3ζ) that assemble as three dimers (εγ, εδ, and ζζ) [1]. The CD3 complex is a marker of T cells [1], which recognizes and participates in the elimination of infected cells and tumor cells through the interaction between the TCR on T cells and the MHC-peptide complex on antigen-presenting cells [2]. Because of its short cytoplasmic tail, the TCR lacks the ability to signal and requires non-covalent association with the CD3 [2]. Upon antigen recognition, the TCR/CD3 complex on T cells triggers downstream intracellular signaling and participates in T cell activation [2]. The CD3ε polypeptide chain (~25 kDa) is targeted by OKT3, a monoclonal antibody that is clinically approved for the induction of immunosuppression in solid organ transplantation [3].
1. Chetty R. & Gatter K., 1994. CD3: structure, function, and role of immunostaining in clinical practice. J. Pathol. 173(4):303-307.
2. Smith-Garvin J.E. et al., 2009. T Cell Activation. Ann. Rev. Immunol. 27:591-619.
3. Norman D.J., 1995. Mechanisms of action and overview of OKT3. Therapeutics Drug Monitoring. 17:615-620.