Murine IgG1e3 Isotype Control

Monoclonal mouse IgG1e3 antibody against E. coli β-galactosidase for in vivo use

Anti-β-Gal-mIgG1e3 InvivoFit™ is a recombinant monoclonal antibody (mAb) comprising the variable region of the hybridoma-derived mAb Mouse IgG2a Control (clone: T9C6) that targets E. coli β-galactosidase (β-Gal).

This mAb does not react with murine proteins. 

Anti-β-Gal-mIgG1e3 InvivoFit™ features the murine IgG1e3 isotype that contains a point mutation D265A (a replacement of aspartic acid by alanine at position 265), resulting in the complete loss of cytolytic effector functions [1, 2].

This mAb is provided as an isotype control for use with InvivoGen's IgG1e3 antibodies in the mouse anti-mouse mAbs collection. 

It has been produced in CHO cells and purified by affinity chromatography with protein G. 

Anti-β-Gal-mIgG1e3 InvivoFit™ is specifically adapted for in vivo use in mice:

• Sequence is 100% murine
• Guaranteed sterile, endotoxin level < 1 EU/mg
• Suitable for parenteral delivery in mice (azide-free)
• Low aggregation < 5%
• Produced in both animal-free facilities and defined media

References:

1. Yamazaki T. et al., 2005. Blockade of B7-H1 on macrophages suppresses CD4+ T cell proliferation by augmenting IFN-gamma-induced nitric oxide production. J Immunol. 175(3):1586-92.
2. Baudino L. et al., 2008. Crucial role of aspartic acid at position 265 in the CH2 domain for murine IgG2a and IgG2b Fc-associated effector functions. J Immunol. 181(9):6664-9.

Specifications

Clonality: Monoclonal antibody

Specificity: E. coli β-galactosidase (β-Gal)

Isotype: Mouse IgG1e3 (D265A mutation; no effector function), kappa

Source: CHO cells

Purity: Purified by affinity chromatography with protein G

Quality control:

• Absence of binding confirmed by flow cytometry
• The complete sequence of this antibody has been verified
• < 5% aggregates (confirmed by size exclusion chromatography)
• Guaranteed sterile and <1 EU/mg (determined by the LAL assay)

Contents

Anti-β-Gal-mIgG1e3 InvivoFit™ is provided sterile, endotoxin-free, azide-free and lyophilized.  

This product is available in two pack sizes:

• bgal-mab15-1​: 1 mg
• bgal-mab15-10​: 10 x 1 mg

Product is shipped at room temperature.

Store lyophilized antibody at -20 °C.

Lyophilized product is stable for at least 1 year.