Murine IgG1e3 Isotype Control

Anti-β-Gal-mIgG1e3 InvivoFit™ Unit size Cat. code Docs Qty Price
β-galactosidase antibody: Mouse IgG1e3 isotype control
1 mg
10 mg
50 mg

Monoclonal mouse IgG1e3 antibody against E. coli β-galactosidase for in vivo use

Anti-β-Gal-mIgG1e3 InvivoFit™ is a recombinant monoclonal antibody (mAb) comprising the variable region of the hybridoma-derived mAb Mouse IgG2a Control (clone: T9C6) that targets E. coli β-galactosidase (β-Gal).

This mAb does not react with murine proteins. 

Anti-β-Gal-mIgG1e3 InvivoFit™ features the murine IgG1e3 isotype that contains a point mutation D265A (a replacement of aspartic acid by alanine at position 265), resulting in the complete loss of cytolytic effector functions [1, 2].

This mAb is provided as an isotype control in an InvivoFit™ grade for use with InvivoGen's IgG1e3 antibodies in the mouse anti-mouse mAbs collection. 

It has been produced in Chinese hamster ovary (CHO) cells and purified by affinity chromatography with protein A. 


Key features of Anti-β-Gal-mIgG1e3 InvivoFit™:

  • Sequence is 100% murine
  • Filter-sterilized (0.2 µm), endotoxin level < 1 EU/mg
  • Suitable for parenteral delivery in mice (azide-free)
  • Low aggregation < 5%
  • Produced in both animal-free facilities and defined media



1. Yamazaki T. et al., 2005. Blockade of B7-H1 on macrophages suppresses CD4+ T cell proliferation by augmenting IFN-gamma-induced nitric oxide production. J Immunol. 175(3):1586-92.
2. Baudino L. et al., 2008. Crucial role of aspartic acid at position 265 in the CH2 domain for murine IgG2a and IgG2b Fc-associated effector functions. J Immunol. 181(9):6664-9.

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Clonality: Monoclonal antibody

Specificity: E. coli β-galactosidase (β-Gal)

Isotype: Mouse IgG1e3 (D265A mutation; no effector function), kappa

Source: CHO cells

Purity: Purified by affinity chromatography with protein A

Formulation: Lyophilized from 0.2 μm filtered solution in 150 mM sodium chloride, 20 mM sodium phosphate buffer with 5% saccharose

Application: Negative control for murinized IgG1e3 antibodies

Quality control:

  • The absence of binding was confirmed by flow cytometry
  • The complete sequence of this antibody has been verified
  • < 5% aggregates (confirmed by size exclusion chromatography)
  • Endotoxin level <1 EU/mg (determined by the LAL assay)
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Anti-β-Gal-mIgG1e3 InvivoFit™ is filter-sterilized (0.2 µm), endotoxin-free, azide-free, and lyophilized.  


This product is available in three pack sizes:

  • bgal-mab15-1​; 1 mg
  • bgal-mab15-10​: 10 mg
  • bgal-mab15-50​: 50 mg (5 x 10mg)


room temperature The product is shipped at room temperature.

store Store lyophilized antibody at -20 °C.

stability Lyophilized product is stable for at least 1 year.

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