NLRP3 inflammasome Inducer - Cholesterol Crystals

Inflammasome activation with cholesterol crystals

Cholesterol crystals are provided ready-to-use to study NLRP3 inflammasome activation, validated using proprietary IL-1β reporter cells.

Cholesterol crystals are the hallmark of atherosclerosis, a condition where plaque builds up in arteries, increasing the risk of heart attacks and strokes [1]. They act as key danger-associated molecular patterns (DAMPs) able to stimulate the NLRP3 inflammasome-dependent induction of pro-inflammatory cytokines, namely interleukin 1β (IL-1β) and IL-18 [1]. This inflammatory response contributes to the progression of atherosclerosis and other life-threatening pathologies, such as Type 2 Diabetes and cancer [1].

More details

Cholesterol crystals for in vitro experiments are typically prepared by precipitating ultrapure cholesterol in 1-propanol, which can lead to significant batch-to-batch variation in potency.

InvivoGen's ready-to-use cholesterol crystals are produced in a controlled environment and functionally tested to ensure consistency. Designed for in vitro use in cell culture, their biological activity has been validated using InvivoGen's THP1-Null2 cells and HEK-Blue™ IL-1β cells (see figure).

InvivoGen also offers:

• HEK-Blue™ IL-1β: IL-1β Sensor Cells
• THP1-HMGB1-Lucia™: Reporter Cells

Key features

Potent inducer of the NLRP3 inflammasome
Each lot is functionally tested for robust and reproducible results

Read our review on the NLRP3 inflammasome.

Download our Practical guide on Inflammasomes.

Reference:

1. Duewell P, et al., 2010. NLRP3 inflammasomes are required for atherogenesis and activated by cholesterol crystals. Nature. 464(7293):1357-611.

Clear allRefined by

Products1

Data Sheet1

2 Search results: tlrl-chol