Notification: Reference #thpd-koikke-av can only be ordered together with reference #thpd-koikke.

IKKε knockout dual reporter monocytes

THP1-Dual™ KO-IKKε cells were generated from the THP1-Dual™ cell line, which is derived from the human THP-1 monocytic cell line, through the stable knockout of the IKKε gene. THP1-Dual™ KO-IKKε cells feature two reporters allowing the study of the IRF pathway, by monitoring the activity of an inducible secreted Lucia luciferase, and the NF-κB pathway by monitoring the activity of an inducible SEAP (secreted embryonic alkaline phosphatase). Lucia luciferase and SEAP activities are readily assessable in the supernatant using QUANTI-Luc™ 4 Lucia/Gaussia and QUANTI-Blue™ Solution detection reagents, respectively.

IKK-related kinases IκB kinase ε (IKKε), also known as IKK-inducible or IKK-i, is an IKK-related kinase. IKKε is highly similar to the other reported IKK-related kinase, TANK binding kinase 1 (TBK1), and both play an essential role in innate immune responses. Notably, their roles and signaling pathways are highly intertwined (i.e. IKKε/TBK1), thus there remain many uncertainties about the individual function of these kinases [1-3]. Therefore, a comparison between THP1‑Dual™ KO‑IKKε and InvivoGen's THP1-Dual™ KO-TBK1 cells allows both overlap and differences in signaling functions between IKKε and TBK1 to be fully elucidated (see figures).

More details

NF-κB and IRF signaling pathways in THP1-Dual™ KO-IKKε cells

Key Features:

Verified knockout of the IKKε gene (PCR, DNA sequencing, Western blot, and functional assays)
Functionally validated with a selection of PRR ligands and cytokines
Readily assessable Lucia luciferase and SEAP reporter activities
The stability for 20 passages, following thawing, has been verified
Guaranteed mycoplasma-free

Applications:

Defining the role of IKKε in PRR-induced signaling, or related cell signaling pathways
Highlighting possible overlap and/or differences between IKKε and TBK1 signaling functions (see figures)
Developing novel IKKε specific inhibitors

References:

1. Chau, T.L. et al. 2008. Are the IKKs and IKK-related kinases TBK1 and IKK-epsilon similarly activated? Trends Biochem Sci 33, 171-180.
2. Clement, J.F. et al. 2008. The IKK-related kinases: from innate immunity to oncogenesis. Cell Res 18, 889-899.
3. Verhelst, K. et al. 2013. IkappaB kinase epsilon (IKKepsilon): a therapeutic target in inflammation and cancer. Biochem Pharmacol 85, 873-880.

Clear allRefined by

Products2

2 Search results: thpd-kotbk