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SAMHD1 knockout NF-κB-SEAP and IRF-Lucia luciferase reporter monocytes

Signaling pathways in THP1-Dual™ KO-SAMHD1 Cells

THP1-Dual™ KO-SAMHD1 cells were generated from THP1-Dual™ cells through the stable biallelic knockout of the SAMHD1 gene. Human THP1 monocytes or derived macrophages are a common cellular model to study DNA sensing as they naturally express all cytosolic DNA sensors identified so far (except DAI). THP1-Dual™ KO-SAMHD1 cells feature two inducible reporter genes, allowing the concomitant study of the IRF and NF-κB pathways, by monitoring the Lucia luciferase and SEAP (secreted embryonic alkaline phosphatase) activities, respectively.

SAMHD1 (sterile alpha motif and histidine-aspartate domain-containing protein 1) is a predominantly nuclear enzyme playing a major role in nucleotide homeostasis by balancing cellular dNTP (deoxynucleoside triphosphate) levels [1]. Furthermore, it can influence viral activity and act as an important negative regulatory factor of the human innate immune response [1-3].

More details

Key features:

Biallelic knockout of the SAMHD1 gene
Functionally validated with a selection of PRR ligands and cytokines
Readily assessable Lucia luciferase and SEAP reporter activities

Applications:

Study of IRF and NF-kB-dependent SAMHD1 signaling pathways
Screening of interactions between SAMHD1 and other signaling protein
Study the role of SAMHD1 in innate immunity, tumorigenesis, or viral replication

1. Deutschmann et al., 2021. SAMHD1 … and Viral Ways around It. Viruses. 2021;13(3):395.
2. Rice et al., 2009. Mutations involved in Aicardi-Goutières syndrome implicate SAMHD1 as a regulator of the innate immune response. Nature genetics, 41(7), 829–832.
3. Oo et al., 2022. Elimination of Aicardi–Goutières syndrome protein SAMHD1 activates cellular innate immunity and suppresses SARS-CoV-2 replication. jbc.2022.101635

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