Notification: Reference #thp-gb1lc-av can only be ordered together with reference #thp-gb1lc.

HMGB1-Lucia reporter assay for pyroptosis and necroptosis monitoring

Signaling in THP1-HMGB1-Lucia™ cells

High mobility group box 1 (HMGB1) plays a critical role in the stress response not only inside the cell as a DNA chaperone and cell death regulator but also outside the cell as a prototypical alarmin. Indeed, during non-apoptotic programmed or regulated cell death, such as pyroptosis and necroptosis, HMGB1 is released along with other pro-inflammatory molecules [1-3].

More details about Pyroptosis and necroptosis

THP1-HMGB1-Lucia™ cells are designed to monitor necroptosis and inflammasome-mediated pyroptosis, two forms of necrotic cell death characterized by the release of the alarmin HMGB1 upon cell membrane rupture [1-3]. These cells are derived from the THP-1 human monocytic cell line and stably express a HMGB1::Lucia-luciferase fusion protein. They respond to commonly used inflammasome inducers and necroptosis cocktail inducers. The release of the HMGB1::Lucia protein in the extracellular milieu upon pyroptosis or necroptosis can be readily measured using QUANTI-Luc™ 4 Lucia/Gaussia, a Lucia and Gaussia luciferase detection reagent.

Key features

Pyroptosis and necroptosis reporter cells
Readily assessable HMGB1::Lucia reporter activity
Guaranteed mycoplasma-free

Levels of IL-1β can be measured by Western blot, ELISA, or using InvivoGen’s HEK-Blue™ IL-1β cellular assay. Alternatively, secreted IL-18 can be detected using InvivoGen’s HEK-Blue™ IL-18 cellular assay.

Download our Practical guide on Inflammasomes

References:

1. Broz P. and Dixit V.M., 2016. Inflammasomes: mechanism of assembly, regulation and signalling. Nat Rev Immunol. 16:407-20.
2. Kovacs S.B. and Miao E.A., 2017. Gasdermins: effectors of pyroptosis. Trends Cell Biol. 27:673-84.
3. Grootjans S. et al., 2017. Initiation and execution mechanisms of necroptosis: an overview. Cell Death Differ. 24:1184-95.

Clear allRefined by

Products1

1 Search results: thp-gb1lc