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Gasdermin D knockout in RAW 264.7 cells

Inflammasome signaling in RAW-ASC KO-GSDMD cells

Gasdermin D (GSDMD) is a cytoplasmic protein with a pore-forming ability that has been described as a major actor in both canonical and non-canonical inflammasome responses [1, 2].

More details about GSDMD

To foster studies on GSDMD, InvivoGen has developed RAW-ASC KO-GSDMD cells, which were generated from the RAW-ASC cell line that derives from the naturally ASC deficient RAW 264.7 macrophage cell line [3]. RAW-ASC KO-GSDMD cells stably express the transfected murine ASC gene and have a stable knockout of the gasdermin D (GSDMD) gene.

• RAW-ASC KO-GSDMD cells – Knockout (KO) of the GSDMD gene and expression of the murine ASC gene

In this cell line, both mature IL-1β secretion and pyroptotic cell death are abolished upon canonical and non-canonical inflammasome activation. This cell line is a useful tool to study the role of GSDMD in the inflammasome responses and is an alternative to the in vitro differentiation of mouse bone-marrow-derived macrophages. Additionally, it can be used as a control cell line for the screening of novel therapeutics that target GSDMD.

FEATURES OF RAW-ASC KO-GSDMD CELLS:

Verified biallelic knockout of the GSDMD gene and stable expression of the ASC gene (Western blot)
Complete abrogation of mature IL-1β secretion and pyroptosis after canonical and non-canonical inflammasome activation

Download our Practical guide on Inflammasomes

References:

1. Feng S. et al., 2018. Mechanisms of Gasdermin family members in inflammasome signaling and cell death. J. Mol. Biol. 430:3068.
2. Kovacs S.B. & Miao E.A. 2017. Gasdermins: effectors of pyroptosis. Trends Cell. Biol. 27:673.
3. Pelerin P. et al., 2008. P2X7 receptor differentially couples to distinct release pathways for IL-1β in mouse macrophage. J. Immunol. 180:7147.

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