Notification: Reference #hkd-tnfa-av can only be ordered together with reference #hkd-tnfa.

TNF-α reporter cells with SEAP and Lucia luciferase double read-out

Signaling pathways in HEK-Blue-Lucia™ TNF-α cells

HEK-Blue-Lucia™ TNF-α* cells were engineered from the human embryonic kidney HEK293 cell line to detect bioactive human tumor necrosis factor-alpha (TNF-α) by monitoring the activation of the NF-κB pathway. In addition, these cells can be used for screening antibodies or small molecule inhibitors targeting the TNF-α pathway.

TNF-α is a multi-functional pro-inflammatory cytokine involved in the regulation of a wide spectrum of biological processes, such as cell proliferation, differentiation, and apoptosis [1].

More details

Cell line description

HEK-Blue-Lucia™ TNF-α cells were generated by stable transfection with the genes encoding for two NF-κB-inducible reporters: secreted embryonic alkaline phosphatase (SEAP) and Lucia luciferase. This feature allows the double readout of the NF-κB actiavtion upon the binding of TNF-α to its receptor, by monitoring the activity of SEAP and Lucia luciferase using QUANTI-Blue™ Solution and QUANTI-Luc™ 4 Lucia/Gaussia, respectively.

HEK-Blue™ TNF-α cells detect human (h) TNF-α. Of note, these cells are not responsive to hIL-1β, and hIFN-β (see figures).

Key features

Fully functional TNF-α signaling pathway
Readily assessable NF-κB-inducible SEAP and Lucia luciferase reporter activities
Strong response to humanTNF-α
No response to human IL-1β

Applications

Detection and quantification of human TNF-α activity
Screening of anti-TNF-α or anti-TNFR1/TNFR2 antibodies
Screening of small molecule inhibitors of the TNF-α pathway

Note: This cell line has been renamed. It was formerly known as "HEK-Dual™ TNF-α". The cat. code (hkd-tnfa) remains unchanged.

Reference:

1.Steeland S, Libert C, Vandenbroucke RE. 2018. A New Venue of TNF Targeting. Int J Mol Sci.;19(5):1442.

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