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STING Knockout IRF Reporter Cell Line

HEK-Blue™ ISG-KO-STING cells were specifically designed to study the STING/TBK1/IRF3 signaling pathway. HEK-Blue™ ISG-KO-STING cells were generated from the HEK-Blue™ ISG cells through the stable knockout of the STING gene.

The parental HEK-Blue™ ISG cells were derived from the PEAKrapid cell line (similar to ATCC® CRL-2828™) which itself was derived from the HEK293 cell line. Unlike their parental cell line, HEK-Blue™ ISG-KO-STING cells do not respond to cytosolic DNA, CDNs, and xanthenone derivatives, such as DMXAA.

HEK-Blue™ ISG-KO-STING cells can detect bioactive type I IFNs through the activation of the JAK-STAT-IRF9 pathway.

HEK-Blue™ ISG-KO-STING cells express a secreted embryonic alkaline phosphatase (SEAP) under the control of the IRF-inducible promoter comprised of five IFN-stimulated response elements (ISRE) fused to an ISG54 minimal promoter.

Levels of SEAP in the supernatant can be easily determined with QUANTI- Blue™, a reagent that turns purple/blue in the presence of SEAP and by reading the OD at 620-655 nm.

References:

1. Ishikawa H. & Barber G., 2008. STING is an endoplasmic reticulum adaptor that facilitates innate immune signalling. Nature 455, 674–678.
2. Burdette DL. et al., 2011. STING is a direct innate immune sensor of cyclic di-GMP. Nature 478(7370):515-8.
3. ;Wu J. et al., 2013. Cyclic GMP-AMP is an endogenous second messenger in innate immune signaling by cytosolic DNA. Science 339(6121):826-30.

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