Notification: Reference #hkb-ifnlv2-av can only be ordered together with reference #hkb-ifnlv2.

Human Type III IFNs Reporter Cells

HEK-Blue™ IFN-λ cells were engineered from the human embryonic kidney HEK 293 cell line to detect bioactive human type III interferons (IFN-λs) by monitoring the activation of the JAK/ISGF3 pathway. In addition, these cells can be used for screening antibodies or small molecule inhibitors targeting the IFN-λ pathway.

Signaling pathway in HEK-Blue™ IFN-λ Cells

The human IFN-λ family comprises four distinct proteins called IFN-λ1 (interleukin-29, IL-29), IFN-λ2 (IL-28A), IFN-λ3 (IL-28B), and the poorly secreted IFN-λ4. IFN-λs are essential to the anti-viral response at epithelial barriers [1, 2].

More details

Cell line description

HEK-Blue™ IFN-λ cells were generated by stable transfection with the genes encoding the human IFN-λ receptor (IFNLR and IL10R chains), STAT2 and IRF9, to obtain a fully active type I IFN signaling pathway. The other genes of the pathway (JAK1, TyK2, and STAT1) are naturally expressed by these cells. HEK-Blue™ IFN-λ cells were also stably transfected with the secreted embryonic alkaline phosphatase (SEAP) reporter under the control of the ISG54 promoter. This promoter comprises IFN-stimulated response elements (ISRE) that are recognized by the ISGF3 (STAT1/STAT2/IRF9) complex. The binding of IFN-λ to its receptor triggers a signaling cascade leading to the activation of ISGF3 and the subsequent production of SEAP. This can be readily assessed in the supernatant using QUANTI-Blue™ Solution, a SEAP detection reagent.

HEK-Blue™ IFN-λ cells respond to human and/or murine IL-28A and IL-28B, and human IL-29. Of note, these cells do not respond to either type I IFNs (IFN-α/β) or type II IFN (IFN-γ) (see figures).

Key Features

Fully functional IFN-λ signaling pathway
Readily assessable ISGF3-inducible SEAP reporter activity
Strong response to human and murine IFN-λ
No response to IFN-α/β (type I IFNs) and IFN-γ (type II IFNs)

Applications

Detection of human and murine IFN-λ
Quantification of IFN-λ activity in biological samples, such as plasma or serum [3]
Screening of anti-IFN-λ and anti-IFNLR antibodies
Screening of small molecule inhibitors of the IFN-λ pathway

Note: A new clone is provided with an improved Type III IFN response. The cat code has been changed accordingly (hkb-ifnlv2).

References:

1. Lazear HM. et al., 2015. Interferon-λ: Immune Functions at Barrier Surfaces and Beyond. Immunity. 43(1):15-28.
2. Lee S. & Baldridge MT., 2017. Interferon- Lambda: A Potent Regulator of Intestinal Viral Infections. Front Immunol. 8:749.
3. Gómez-Bañuelos E, et al., 2024. Uncoupling interferons and the interferon signature explain clinical and transcriptional subsets in SLE. medRxiv. 2023.08.28.23294734

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