Notification: References #hkb-il1920-av and #hkb-il20-av can only be ordered together with references #hkb-il1920 and #hkb-il20, respectively.

IL-19, IL-20, IL-22, and IL-24 sensing
in HEK-Blue™-derived cells

IL-19, IL-20 and IL-24 Reporter Cells

HEK-Blue™ IL-19/IL-20 and HEK-Blue™ IL-20 reporter cells were engineered from the human embryonic kidney HEK 293 cell line to detect bioactive interleukin 19 (IL-19), IL-20, IL-22, and IL-24 by monitoring the activation of the JAK/STAT3 pathway:

— HEK-Blue™ IL-19/IL-20 cells expressing the IL-20 type I receptor for the detection of human and murine IL-19, IL-20, and IL-24

— HEK-Blue™ IL-20 cells expressing the IL-20 type II receptor for the detection of human and murine IL-20, IL-22, and IL-24

IL-19, IL-20, IL-22, and IL-24 are closely related cytokines that belong to the IL-20 subfamily and play an important role in the protection of barrier tissues. They share the heterodimeric IL-20 type I and type II receptors composed of the IL-20Rα and IL-20Rβ chains, and IL-22Rα1 and IL-20Rβ chains, respectively [1, 2].

More details

Cell Line description:

HEK-Blue™ IL-19/IL-20 and HEK-Blue™ IL-20 cells were generated by stable transfection with the genes encoding for the human IL-20 type I receptor IL-20Rα and IL-20Rβ chains, or type II receptor IL-20Rβ and IL-22Rα chains, respectively. Both cell lines also feature a stable expression of human STAT3 to obtain a fully functional IL-19, IL-20, or IL-24 signaling pathway as well as a STAT3-inducible secreted embryonic alkaline phosphatase (SEAP) reporter. The binding of IL-19, IL-20, IL-22, or IL-24 to their receptor triggers a signaling cascade leading to the activation of STAT3 and the subsequent production of SEAP. This can be readily assessed in the supernatant using QUANTI-Blue™ Solution, a SEAP detection reagent.

— HEK-Blue™ IL-19/IL-20 cells, expressing the IL-20Rα and IL-20Rβ chains (type I receptor), respond to IL-19, IL-20, or IL-24 stimulation. They also respond to IL-27 and IFN-γ. They do not respond to IL-6, IL-10, IL-22, IL-26, and IFN-α/β (see figures).

— HEK-Blue™ IL-20 cells, expressing the IL-22Rα and IL-20Rβ chains (type II receptor), respond to IL-20, IL-22, or IL-24 stimulation. They also respond to IL-27 and IFN-γ. They do not respond to IL-6, IL-10, IL-19, IL-26, and IFN-α/β (see figures).

Key features:

Fully functional IL-19, IL-20, IL-22, and IL-24 signaling pathways
Readily assessable STAT3-inducible SEAP reporter activity

Applications:

Detection of human and murine IL-19, IL-20, and IL-24 using HEK-Blue™ IL-19/IL-20 cells
Detection of human and murine IL-20, IL-22, and hIL-24 using HEK-Blue™ IL-20 cells
Screening of anti-IL-19, anti-IL-20, anti-IL-22, and anti-IL-24 antibodies

References:

1. Ouyang W. et al., 2019. IL-10 family cytokines IL-10 and IL-22: from basic science to clinical translation. Immunity. 50:871-891.
2. Wei H. et al., 2019. Interleukin-10 family cytokines: Immunobiology and structure. Adv Exp Med Biol. 1172:79-96.

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