Colorimetric-based assay reagent for the detection of acid phosphatase 5 (ACP5)

QUANTI-Star™ is a liquid formulation of a colorimetric enzyme assay. It has been developed to determine the release of acid phosphatase 5 (ACP5) in a biological sample, such as cell culture supernatants.

Description

ACP5, also known as tartrate-resistant acid phosphatase (TRAP or TRAPase) is a metalloprotein enzyme, usually located in the lysosome. InvivoGen has developed a secreted (s) version of ACP5 (sACP5) that can be used as a reporter gene.

The level of sACP5 activity in the cell culture supernatant is easily quantified using QUANTI-Star™. This easy-to-use detection reagent contains an ACP5-specific colorimetric substrate, which harbors a phosphate group. Cleavage of this phosphate group by secreted (s)ACP5 leads to the formation of a soluble yellow reaction product, which can be detected at 405 nm using a microplate reader or spectrophotometer. It is therefore combinable with other reporter genes, such as SEAP or Lucia luciferase. ACP5 secretion can be assessed without disturbing cells, thus allowing the repeated sampling of cell cultures for kinetic studies.

For your convenience, InvivoGen offers secreted ACP5 and Lucia-expressing HEK-Lucia-Star™ ARE cells designed to monitor oxidative stress responses.

Features and advantages of QUANTI-Star™ ACP5 Detection reagent

Convenient, easy, and rapid
Cost-effective (1ml = 10 x 96-well plates)
Short hands-on-time < 10 min
Easy to prepare: just mix QS reagent and QS buffer with water
Highly sensitive
Allows for high-throughput screening (HTS)

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