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Poly(I:C) HMW

Poly(I:C) (HMW) Unit size Cat. code Docs Qty Price
Long synthetic analog of dsRNA
10 mg
50 mg
tlrl-pic
+-
$171.00

TLR3 agonist - Polyinosine-polycytidylic acid High molecular weight

Polyinosinic-polycytidylic acid (usually abbreviated as poly(I:C) or poly(rI):poly(rC)) is a synthetic analog of double-stranded RNA (dsRNA), a molecular pattern associated with viral infection. Poly(I:C) activates the antiviral pattern recognition receptors TLR3, RIG-I/MDA5 and PKR, thereby inducing signaling via multiple inflammatory pathways, including NF-kB and IRF.

High Molecular Weight Poly(I:C) comprises long strands of inosine poly(I) homopolymer annealed to strands of cytidine poly(C) homopolymer. The average size of Poly(I:C) HMW is from 1.5 kb to 8 kb.

InvivoGen poly(I:C) HMW is a highly referenced as potent inducer of IFN both in vitro and in vivo.

Poly(I:C) HMW is functionally validated for TLR3 potency using HEK-Blue™ TLR3 Cells. It is tested in TLR2 and TLR4 cellular assays to confirm the absence of microbial contaminants (e.g. endotoxin or bacterial-membrane components).

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Specifications

CAS number: 31852-29-6

Working concentration: 30 ng- 10 μg/ml

Endotoxin level:

Solubility: 5 mg/ml in physiological water (NaCl 0.9%) heated for 10 minutes at 65 - 70°C

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Contents

Poly(I:C) is provided lyophilized and is available in two quantities:

  • tlrl-pic: 10 mg
  • tlrl-pic-5: 50 mg

Sterile endotoxin-free physiological water (NaCl 0.9%), 10 ml is provided with catalog #tlrl-pic and 2 x 25 ml with catalog #tlrl-pic-5

Poly(I:C) is shipped at room temperature

Upon receipt it should be stored at 4°C.

Upon resuspension, prepare aliquots of Poly(I:C) and store at -20°C for long term storage. Store at 4°C for short term storage.

Lyophilized product is stable 1 year at 4°C when properly stored. Resuspended product is stable 1 month at 4°C and 1 year at -20°C.

Avoid repeated freeze-thaw cycles.

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Description

Polyinosinic-polycytidylic acid (poly(I:C)) is a synthetic analog of double stranded RNA (dsRNA), a molecular pattern associated with viral infection.

Both natural and synthetic dsRNAs are known to induce type I interferons (IFN) and other cytokines production. Poly(I:C) is recognized by Toll-like receptor 3 (TLR3) [1, 2].

Upon poly(I:C) recognition, TLR3 activates the transcription factor interferon regulatory factor 3 (IRF3), through the adapter protein Toll-IL-1 receptor (TIR) domain-containing adapter inducing IFN-β (TRIF, also known as TICAM-1) [3].

Activation of IRF3 leads to the production of type I IFNs, especially IFN-β. A second pathway involves the recruitment of TNF receptor-associated factor 6 (TRAF6) or receptor interacting protein 1 (RIP1), with the subsequent activation of the transcription factors NF-κB and AP-1 [4].

Activation of this pathway triggers the production of inflammatory cytokines and chemokines such as TNF-α, IL-6 and CXCL10. Poly(I:C) is also recognized by the cytosolic RNA helicases retinoic acid-inducible protein I (RIG-I) and melanoma differentiation-associate gene 5 (MDA-5) [5].

 

1. Alexopoulou L. et al., 2001. Recognition of double-stranded RNA and activation of NFkappaB by Toll-like receptor 3. Nature, 413(6857):732-8.
2. Matsumoto M. et al., 2002. Establishment of a monoclonal antibody against human Toll-like receptor 3 that blocks double- stranded RNA-mediated signaling. BBRC, 293(5):1364-9.
3. Yamamoto M. et al., 2003. Role of Adaptor TRIF in the MyD88-Independent Toll-Like Receptor Signaling Pathway. Science 301: 640.
4. Kawai T & Akira S., 2008. Tolllikereceptor and RIG-I-like receptor signaling. Ann N Y Acad Sci. 1143:1-20. Review.
5. Kato H. et al., 2006. Differential roles of MDA5 and RIG-I helicases in the recognition of RNA viruses. Nature. 441(7089):101-5.

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Citations

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