2'3'-cGAMP
2’3’-cGAMP | Unit size | Cat. code | Docs | Qty | Price |
---|---|---|---|---|---|
Mammalian (non-canonical) CDN, cyclic [G(2’,5’)pA(3’,5’)p] |
200 µg 500 µg 1 mg 5 mg |
tlrl-nacga23-02 |
You may also need : H-151 | View more associated products ▼
STING ligand
2’3’-cGAMP (cyclic [G(2’,5’)pA(3’,5’)p]) is produced in mammalian cells by cGAS (cGAMP synthase) in response to double-stranded DNA in the cytoplasm.
2’3’-cGAMP is also referred to as “noncanonical“ cGAMP due to the presence of the atypical 2’-5’ phosphodiester linkage between the guanosine and the adenosine.
Similar to the canonical 3’3’-cGAMP, 2’3’-cGAMP serves as a second messenger to activate innate immune responses by binding to STING (stimulator of IFN genes) and subsequently inducing the TBK1-IRF3-dependent production of IFN-β [1].
Structural and functional studies revealed that noncanonical 2’3’-cGAMP is distinct from the canonical 3’3’-cGAMP produced by bacteria [2,3]. 2'3'-cGAMP binds to STING with a much greater affinity than 3'3'-cGAMP [1].
Certain variants of STING are able to distinguish between noncanonical and canonical cGAMP [2].
Read our review on STING: Deciphering the STING Paradox
References
1. Zhang X. et al., 2013. Cyclic GMP-AMP containing mixed phosphdiester linkages is an endogenous high-affinity ligand for STING. Mol Cell.51(2):226-35.
2. Diner E. et al., 2013. The Innate Immune DNA Sensor cGAS Produces a Noncanonical Cyclic Dinucleotide that Activates Human STING. Cell Rep. 3(5):1355-61.
3. Gao P. et al., 2013. Cyclic [G(2',5')pA(3',5')p] is the metazoan second messenger produced by DNA-activated cyclic GMP-AMP synthase. Cell. 153(5):1094-107.
Specifications
Description: STING agonist
Synonym: Cyclic [G(2’,5’)pA(3’,5’)p], cyclic GMP-AMP; c-GpAp sodium salt
CAS number: 1441190-66-4
Formula: C20H22N10O13P2 •2Na
Molecular weight: 718.38
Purity: ≥ 95% by LC/MS
Solubility: 50 mg/ml in water
Quality control:
- The biological activity has been confirmed using cellular assays.
- The absence of endotoxins and other bacterial contaminants has been confirmed using HEK-Blue™ TLR4 and HEK-Blue™ TLR2 cells.
Contents
2’3’-cGAMP is provided as a lyophilized powder, the product is sterile filtered prior to lyophilization.
2’3’-cGAMP is available in three quantities:
- 200 µg 2’3’-cGAMP + 1.5 ml endotoxin-free water.
- 500 µg 2’3’-cGAMP + 1.5 ml endotoxin-free water.
- 1 mg (2 x 500 µg) 2’3’-cGAMP + 1.5 ml endotoxin-free water.
- 5 mg (5 x 1 mg) 2’3’-cGAMP + 10 ml endotoxin-free water.
2’3’-cGAMP is shipped at room temperature
2’3’-cGAMP should be stored at -20°C.