RAW-Lucia™ ISG Cells

RAW-Lucia™ ISG cells were generated from the murine RAW 264.7 macrophage cell line by stable integration of an interferon regulatory factor (IRF)-inducible Lucia luciferase reporter construct.
RAW 264.7 have been reported to express IFI16 [1], DDX41 and AIM2 [2].

RAW-Lucia™ ISG cells express the Lucia luciferase gene under the control of an ISG54 minimal promoter in conjunction with five IFN-stimulated response elements. Thus, RAW-Lucia™ ISG cells allow the monitoring of IRF activation by determining the activity of Lucia luciferase.
The levels of IRF-induced Lucia luciferase in the cell culture supernatant can be easily monitored using QUANTI-Luc™, a luciferase detection reagent. RAW-Lucia™ ISG cells are resistant to Zeocin™.

RAW-Lucia™ ISG cells are responsive to murine IFN-α and IFN-β but do not respond to their human counterparts. They are also responsive to PRR ligands that trigger the IFN signaling pathway, including CDS ligands, such as transfected double stranded DNA, RIG-I ligands, such as transfected double stranded RNA, and STING ligands, such as cGAMP.

Figures for this product

IRF response of THP1-Lucia™ ISG and RAW-Lucia™ ISG cells


Antibiotic resistance: Zeocin™

Growth medium: DMEM medium, 2 mM L-glutamine, 10% FBS supplemented with 100 µg/ml Normocin™ and 200 µg/ml Zeocin™.

Guaranteed mycoplasma-free

Shipped on dry ice

These products are covered by a Limited Use License (See Terms and Conditions).


1 vial containing 5-7 x 106 cells

100 μl Zeocin™ (100 mg/ml)

1 ml Normocin™ (50 mg/ml)

1 pouch of QUANTI-Luc™ (100 ml)


1. Kawasaki T. et al., 2011. Recognition of nucleic acids by pattern-recognition receptors and its relevance in autoimmunity. Immunol Rev. 243(1):61-73.
2. Stein SC & Falck-Pedersen E., 2012. Sensing adenovirus infection: activation of interferon regulatory factor 3 in RAW 264.7 cells. J Virol. 86(8):4527-37.


Recent articles using InvivoGen RAW-Lucia™ ISG Cells


RAW-Lucia™ ISG Cells

Description Mouse Macrophages - CDS Reporter Cells
Cat. Coderawl-isg
Unit Size3-7 x 10e6 cells
Price For price or distributor address,
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