RAW-Lucia™ ISG-KO-cGAS Cells

cGAS Knockout IRF-Inducible Lucia luciferase reporter mouse macrophages

RAW-Lucia™ ISG-KO-cGAS cells were generated from the RAW-Lucia™ ISG cell line, which is derived from the murine RAW 264.7 macrophage cell line, through the stable knockout of the cGAS gene.
RAW-Lucia™ ISG-KO-cGAS cells express a secreted reporter gene, Lucia luciferase, under the control of the I-ISG54 promoter which is comprised of the IFN-inducible ISG54 promoter enhanced by a multimeric ISRE.

RAW 264.7 have been reported to express several CDSs, including cGAS [1]. RAW-Lucia™ ISG-KO-cGAS cells allow the monitoring of IRF activation by determining the activity of Lucia luciferase. The levels of IRF-induced Lucia in the cell culture supernatant can be easily monitored using QUANTI-Luc™, a Lucia luciferase detection reagent.

RAW-Lucia™ ISG-KO-cGAS cells respond to interferons (e.g. IFN-α and IFN-β), cyclic dinucelotides (e.g. 2'3'-cGAMP and c-di-GMP) and transfected poly(I:C). However, as expected, they respond very poorly to transfected DNA, such as poly(dA:dT)/LyoVec and VACV70/LyoVec.

RAW-Lucia™ ISG-KO-cGAS cells are resistant to Zeocin™.

Figures for this product

IRF induction in RAW-Lucia™ ISG-KO-cGAS


Antibiotic resistance: Zeocin™

Growth Medium: DMEM, 4.5 g/l glucose, 10% fetal bovine serum (FBS), 100 µg/ml Normocin™, 2 mM L-glutamine

Quality Control: cGAS knockout is verified by functional assays and DNA sequencing to confirm frameshift mutation/deletion. The cells are guaranteed mycoplasma-free.

This product is covered by a Limited Use License (See Terms and Conditions).


1 vial containing 3-7 x 106 cells

100 μl Zeocin™ (100 mg/ml)

1 ml Normocin™ (50 mg/ml)

1 pouch of QUANTI-Luc™ (Luciferase detection medium)

Shipped on dry ice


1. Lam E. et al., 2014. Adenovirus Detection by the cGAS/STING/TBK1 DNA Sensing Cascade. J Virol. 88(2):974-81.


RAW-Lucia™ ISG-KO-cGAS Cells

Description Murine RAW 264.7 macrophages - cGAS Knockout IRF-reporter cells
Cat. Coderawl-kocgas
Unit Size3-7 x 10e6 cells
Price For price or distributor address,
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