RAW-Blue™ Cells

RAW-Blue™ cells are derived from the murine RAW 264.7 macrophages with chromosomal integration of a secreted embryonic alkaline phosphatase (SEAP) reporter construct inducible by NF-κB and AP-1. RAW-Blue™ cells express many pattern-recognition receptors (PRRs), including toll-like receptors (TLRs), NOD-like receptors (NLRs), RIG-I-like receptors (RLRs) and C-type lectin receptors (CLRs). Therefore, RAW-Blue™ cells can be used as:

- TLR reporter cells: they express all TLRs, with the exception of TLR5, at different levels

- NOD reporter cells: they express NOD2 and, to a lesser extent, NOD1

- RLR reporter cells: they express RIG-I and MDA-5

- CLR reporter cells: they express Dectin-1

The presence of agonists of these PRRs induces signaling pathways leading to the activation of NF-κB and AP-1, and the subsequent production of SEAP. Levels of SEAP can be easily monitored using the detection medium QUANTI-Blue™.


Figures for this product

TLR and NOD stimulation profile in RAW-Blue™ CellsResponse of RAW-Blue™ cells to Dectin-1 and TLR2 agonists


Antibiotic resistance: G418, Zeocin™

Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™ supplemented with Zeocin™ selective antibiotic only

Guaranteed mycoplasma-free

Shipped on dry ice


1 vial containing 3-7 x 106 cells

100 μl Zeocin™ (100 mg/ml)

1 ml Normocin™ (50 mg/ml)

1 pouch of QUANTI-Blue™ (100 ml)


Expression of PRRs mRNA in RAW-blue cells

Expression of TLR, RLR and NOD mRNAs in RAW-Blue™ cells determined by RT-PCR. RT-PCRs were performed using InvivoGen’s Mouse TLR RT-PCR Primer Set, NOD and RLR RT-Primer Pairs.


RAW-Blue™ cells

Cat. Coderaw-sp
Unit Size3-7 x 10e6 cells
Price For price or distributor address,
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