RAW-Lucia™ ISG-KO-MAVS CellsNEW!

MAVS Knockout IRF-Lucia Luciferase Reporter Cell Line

RAW-LuciaISG-KO-MAVS cells were generated from RAW-Lucia™ ISG cells through the stable knockout of the MAVS gene. These cells derive from the murine RAW 264.7 macrophage cell line, which has been reported to express many pattern recognition receptors (PRRs), including the dsRNA sensors MDA-5 [1] and RIG-I [2, 3] along with their adaptor protein MAVS [3].

RAW-LuciaISG-KO-MAVS and RAW-LuciaISG cells can be used to study the role of MAVS by monitoring of IRF-induced Lucia luciferase activity. They express the gene for secreted Lucia luciferase under the control of an IFN-inducible IFN-stimulated genes 54 (ISG54) promoter enhanced by a multimeric IFN-stimulated response elements (ISRE). The levels of IRF-induced Lucia in the cell culture supernatant can be easily monitored using QUANTI-Luc™a Lucia luciferase detection reagent.

RAW-LuciaISG-KO-MAVS cells are resistant to Zeocin™.

Figures for this product

IRF INDUCTION (Lucia luciferase reporter)Figure 1B: IRF INDUCTION (Lucia luciferase reporter)

Specifications

Antibiotic resistance: Zeocin™

Growth Medium: DMEM, 4.5 g/l glucose, 10% fetal bovine serum (FBS), 100 µg/ml Normocin™, 2 mM L-glutamine

Quality Control: MAVS knockout has been verified by functional assays (see validation sheet) and DNA sequencing. The stability of this cell line for 20 passages following thawing has been verified. The cells are guaranteed mycoplasma-free.

This product is covered by a Limited Use License (See Terms and Conditions).

Contents

1 vial containing 3-7 x 106 cells

100 μl Zeocin™ (100 mg/ml)

1 ml Normocin™ (50 mg/ml)

1 pouch of QUANTI-Luc™ (Luciferase detection medium)

Shipped on dry ice

Description

RAW-Lucia ISG KO-MAVS stimulation pathway

Mitochondrial antiviral-signaling protein (MAVS; also known as IPS1, CARDIF, VISA) is an adaptor protein that plays a critical role in the immune response to viral infection. The innate immune system senses intracellular double-stranded RNA (dsRNA), a replication intermediate for RNA viruses, through two RNA helicases: retinoic acid inducible gene-I (RIG-I) and melanoma differentiation-association gene 5 (MDA5). These two sensors recognize different ligands, yet both signal through MAVS. Specifically, upon recognition of dsRNA, they are recruited by MAVS to the outer membrane of the mitochondria leading to the activation of interferon regulatory factor 3 (IRF3), which in turn regulates the expression of type I interferons (IFNs).

Ref.

1. Hasan M. et al., 2011. Antimicrobial peptides inhibit polyinosinic-polycytidylic acid-induced immune responses. J Immunol. 187(11):5653-9.

2. Melchjorsen J. et al., 2005.Activation of innate defense against a paramyxovirus is mediated by RIG-I and TLR7 and TLR8 in a cell-type-specific manner. J Virol. 79:12944-51.

3. Yamashita M. et al., 2013. Antiviral innate immunity disturbs podocyte cell function. J Innate Immun. 5:231-41.

ORDERING

RAW-Lucia™ ISG-KO-MAVS CellsNEW!

Description MAVS Knockout IRF-Lucia Luciferase Reporter Cells
Cat. Code rawl-komavs
Unit Size 3-7 x 10e6 cells
Price For price or distributor address,
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