RAW-Lucia™ ISG-KO-TBK1 Cells

TBK1 Knockout IRF-Inducible Lucia luciferase reporter mouse macrophages

RAW-Lucia™ ISG-KO-TBK1 cells were generated from the RAW-Lucia™ ISG cell line, which is derived from the murine RAW 264.7 macrophage cell line, through the stable knockout of the TBK1 gene. TBK1  (TANK-Binding Kinase 1) functions as a key node protein in several cell signaling pathways, including innate immune response, cell growth and proliferation.

RAW-Lucia™ ISG-KO-TBK1 and RAW-Lucia™ ISG cells express a secreted reporter gene, Lucia luciferase, under the control of the I-ISG54 promoter which is comprised of the IFN-inducible ISG54 promoter enhanced by a multimeric ISRE.

RAW-Lucia™ ISG-KO-TBK1 and RAW-Lucia™ ISG cells can be used to study the role of TBK1 by monitoring IRF-induced Lucia luciferase activity. The levels of IRF-induced Lucia in the cell culture supernatant can be easily monitored using QUANTI-Luc™, a Lucia luciferase detection reagent.

The response of RAW‑Lucia™ ISG-KO-TBK1 cells to murine type I interferons (IFNs) is unaffected by the knockout of the TBK-1 gene. As expected, RAW‑Lucia™ ISG-KO-TBK1 cells do not respond to cyclic dinucleotides (e.g. 2'3'-cGAMP and c-di-AMP) and to transfected double-stranded DNA, such as poly(dA:dT)/LyoVec and VACV70/LyoVec.

RAW-Lucia™ ISG-KO-TBK1 cells are resistant to Zeocin™.

Figures for this product

IRF induction in RAW-Lucia™ ISG-KO-TBK1


Antibiotic resistance: Zeocin™

Growth Medium: DMEM, 4.5 g/l glucose, 10% fetal bovine serum (FBS), 100 µg/ml Normocin™, 2 mM L-glutamine

Quality Control: TBK1 knockout is verified by functional assays and DNA sequencing to confirm frameshift mutation/deletion. The cells are guaranteed mycoplasma-free.

This product is covered by a Limited Use License (See Terms and Conditions).


1 vial containing 3-7 x 106 cells

100 μl Zeocin™ (100 mg/ml)

1 ml Normocin™ (50 mg/ml)

1 pouch of QUANTI-Luc™ (Luciferase detection medium)

Shipped on dry ice


RAW-Lucia™ ISG-KO-TBK1 Cells

Description Murine RAW 264.7 macrophages - TBK1 Knockout IRF-reporter cells
Cat. Coderawl-kotbk
Unit Size3-7 x 10e6 cells
Price For price or distributor address,
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