HEK-Blue™ KD-TLR5 cells

TLR5 deficient, IL-1β reporter cells

HEK-Blue™ KD-TLR5 cells are designed to monitor bioactive IL-1β secreted by THP-1 cells upon flagellin-induced NLRC4 activation.

HEK-Blue™ KD-TLR5 cells are derived from the HEK293 cell line, which endogenously expresses TLR5 and the IL-1β receptor (IL-1R).
This cell line features an NF-κB-inducible SEAP reporter gene and was engineered to knock-down the expression of TLR5 to avoid activation of NF-κB upon flagellin-induced TLR5 stimulation.

The knockdown of TLR5 permits the analysis of flagellin specifically for its NLRC4 stimulating activity.
Binding of IL-1β to IL-1R initiates a signaling cascade leading to the activation of NF-κB and the subsequent production of SEAP. Detection of SEAP in the supernatant of HEK-Blue™ KD-TLR5 cells can be readily assessed using the QUANTI-Blue™ assay.

Figures for this product

Detection of flagellin-induced IL-1β using HEK-Blue™KD-TLR5 cells.


Antibiotic resistance: Zeocin™, puromycin

Growth Medium: DMEM, 4.5 g/l glucose, 2 mM L-Glutamine, 10% (v/v) heat-inactivated fetal bovine serum (30 min at 56ºC), 50 U/ml penicillin, 50 µg/ml streptomycin, 100 µg/ml Normocin™

Guaranteed mycoplasma-free

Shipped on dry ice


• 1 vial of HEK-Blue™ KD-TLR5 cells (3-7 x 106 cells) in freezing medium.
• 100 μl Zeocin™ at 100 mg/ml.
• 100 μl puromycin at 10 mg/ml. S
• 1 ml Normocin™ (50 mg/ml). Normocin™ is a formulation of three antibiotics active against mycoplasmas, bacteria and fungi.
• 1 pouch of QUANTI-Blue™ (SEAP detection medium).

IMPORTANT: Cells are shipped frozen. If cells are not frozen upon arrival, contact InvivoGen immediately.


Quality Control

TLR5 knockdown is verified by functional assays and qRT-PCR.
These cells are guaranteed mycoplasma-free.


HEK-Blue™ KD-TLR5 cells are designed to monitor bioactive IL-1β secreted by THP-1 cells upon flagellin-induced NLRC4 activation.
Levels of IL-1β secreted into the supernatant of THP-1 cells can be monitored using the HEK-Blue™ KD-TLR5 cell line. THP1-NLRC4 and HEK-Blue™ KD-TLR5 cell lines can be used sequentially (option 1) or co-cultured to save time (option 2).

THP-1/HEK-Blue™ IL-1β Assay :

Detection of IL-1beta in THP-1 supernatants



HEK-Blue™ KD-TLR5 cells

Description TLR5 deficient - IL1-β SEAP reporter HEK293 cells
Cat. Codehkb-kdtlr5
Unit Size3-7 x 10e6 cells
Price For price or distributor address,
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Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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