HEK-Lucia™ RIG-I CellsNEW

HEK-LuciaRIG-I cells were generated from HEK-LuciaNull cells, HEK293-derived cells that stably express the secreted Lucia luciferase reporter gene. This reporter gene is under the control of an IFN‑inducible ISG54 promoter enhanced by a multimeric IFN‑stimulated response elements (ISRE). HEK-Lucia™ RIG-I cells stably express high levels of human RIG-I and respond strongly to cytosolic RIG-I ligands such as 3p-hpRNA and 5’ppp‑dsRNA (see validation sheet).

HEK-LuciaRIG-I and HEK-LuciaNull cells can be used to study the role of RIG-I by monitoring IRF-induced Lucia luciferase activity. The levels of IRF-induced Lucia in the cell culture supernatant can be easily monitored using QUANTI-Luc™, a Lucia luciferase detection reagent.

Note: HEK-LuciaRIG-I and HEK-Lucia™ Null cells endogenously express NOD1, TLR3 and TLR5.

HEK-LuciaRIG-I cells are resistant to blasticidinG418hygromycin, and  Zeocin™. They should be maintained in growth medium supplemented with blasticidin and Zeocin™.

Figures for this product

IRF INDUCTION (Lucia luciferase reporter)

Specifications

Antibiotic resistance: blasticidin, G418, hygromycin, and  Zeocin™

Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) fetal bovine serum (FBS), 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™

Quality Control:

  • The expression of the human RIG-I gene has been confirmed by qRT-PCR.
  • The reporter activity has been validated by stimulating these cells with various RIG-I agonists.
  • The cell line stability for 20 passages following thawing has been verified.
  • HEK-Lucia™ RIG-I cells are guaranteed mycoplasma-free.

Shipped on dry ice

Contents

1 vial of HEK-Lucia™ RIG-I cells (3-7 x 106 cells)

100 μl Zeocin™ (100 mg/ml)

100 μl blasticidin (10 mg/ml)

1 ml Normocin™ (50 mg/ml)

1 pouch of QUANTI-Luc™ (Lucia luciferase detection medium)

 

Details

RIG-I (retinoic-acid-inducible protein 1, also known as Ddx58) receptor is a cytoplasmic RNA helicase that is critical for host antiviral responses. It senses double-stranded RNA (dsRNA), a replication intermediate for RNA viruses, leading to production of type I interferons (IFNs) in infected cells [1]. Notably, RIG-I detects viral RNAs that exhibit an uncapped 5’-di/triphosphate end and a short blunt-ended double stranded (ds) portion [2]. Upon activation, it is recruited by the adaptor MAVS (also known as IPS-1, CARDIF or VISA) to the outer membrane of the mitochondria leading to the activation of several transcription factors including interferon regulatory factor 3 (IRF3), IRF7 and NF-κB [3]. Activated IRF3 and IRF7 bind to IFN-stimulated response elements (ISRE) in the promoters of IFN-stimulated genes (ISG) leading to a type I IFN‑mediated immune response.

Ref.

  1. Gebhardt A. et al., 2017. Discrimination of Self and Non-Self Ribonucleic Acids. Journal of Interferon & Cytokine Research 37: 184-97.
  2. Pichlmair A. et al., 2006. RIG-I-mediated antiviral responses to single-stranded RNA bearing 5’-phosphates. Science 314:997-1001.
  3. Kawai T. et al., 2005. IPS-1, an adaptor triggering RIG-I- and Mda5-mediated type I interferon induction. Nat Immunol. 6(10):981-988.

ORDERING

HEK-Lucia™ RIG-I CellsNEW!

Description Lucia luciferase reporter HEK293 cells expressing human RIG-I
Cat. Codehkl-hrigi
Unit Size3-7 x 10e6 cells
Price For price or distributor address,
please select your country
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