LyoVec™
LyoVec™ is the first lyophilized cationic lipid-based transfection reagent belonging to the new family of phosphonolipids originally described by Floch et al., as efficient transfection reagents both in vitro and in vivo [1-2]. Their positive charge enables them to bind to DNA, and their phospholipid structure promotes fusion with cellular membranes for DNA delivery.
The major constituent of LyoVec™ is the phosphonolipid DTCPTA, which is coupled with DiPPE, a neutral lipid that helps destabilizing membrane bilayers, therefore increasing the in vitro transfection efficiency of LyoVec™.
Specifications
Hands-on time: No more than 15 min. No preparation required the day prior transfection
Transfects with high efficiencies a broad spectrum of mammalian cells
Effective for transient and stable transfections
Works in the presence of serum. No need to wash cells after transfection
Long shelf-life of plasmid DNA (pDNA)/ LyoVec™ complexes
Shipped at room temperature
Documents
Contents
LyoVec™ is provided as a lyophilized powder, sterile and packaged in sealed 2 ml glass vials in different formats:
- 4 vials (sufficient to perform 160 reactions)
- 9 vials (sufficient to perform 360 reactions)
Each order of LyoVec™ comes with 1 vial of control LyoVec™/GFPLacZ complex.
Ref.
1. Floch et al. 1997. Cationic phosphonolipids as non viral vectors for DNA transfection in hematopoietic cell lines and CD34+ cells. Blood Cells Molec.& Diseases 23:69-87.
2. Guillaume-Gable et al. 1998. Cationic phosphonolipids as nonviral gene transfer agents in the lung of mice. Hum Gene Ther 9:2309-2319.
ORDERING
LyoVec™
| Cat. Code | lyec-12 |
|---|---|
| Unit Size | 8 ml, 160 reactions |
| Price | For price or distributor address, please select your country |
LyoVec™
| Cat. Code | lyec-22 |
|---|---|
| Unit Size | 18 ml, 360 reactions |
| Price | For price or distributor address, please select your country |
