LyoVec™

LyoVec™ is the first lyophilized cationic lipid-based transfection reagent belonging to the new family of phosphonolipids originally described by Floch et al., as efficient transfection reagents both in vitro and in vivo [1-2]. Their positive charge enables them to bind to DNA, and their phospholipid structure promotes fusion with cellular membranes for DNA delivery.

The major constituent of LyoVec™ is the phosphonolipid DTCPTA, which is coupled with DiPPE, a neutral lipid that helps destabilizing membrane bilayers, therefore increasing the in vitro transfection efficiency of LyoVec™.

Specifications

Hands-on time: No more than 15 min. No preparation required the day prior transfection

Transfects with high efficiencies a broad spectrum of mammalian cells

Effective for transient and stable transfections

Works in the presence of serum. No need to wash cells after transfection

Long shelf-life of plasmid DNA (pDNA)/ LyoVec™ complexes

Contents

LyoVec™ is provided as a lyophilized powder, sterile and packaged in sealed 2 ml glass vials in different formats:

- lyec-1 : 5 vials (sufficient to perform 200 reactions)

- lyec-2 : 10 vials (sufficient to perform 400 reactions)

lyec-12 : 4 vials (sufficient to perform 160 reactions) and 1 vial of control LyoVec™-GFP/SEAP complex.

lyec-22 : 9 vials (sufficient to perform 360 reactions) and 1 vial of control LyoVec™-GFP/SEAP complex.

Shipped at room temperature

Ref.

1. Floch et al. 1997. Cationic phosphonolipids as non viral vectors for DNA transfection in hematopoietic cell lines and CD34+ cells. Blood Cells Molec.& Diseases 23:69-87.
2. Guillaume-Gable et al. 1998. Cationic phosphonolipids as nonviral gene transfer agents in the lung of mice. Hum Gene Ther 9:2309-2319.

ORDERING

LyoVec™

Cat. Codelyec-12
Unit Size8 ml, 160 reactions
Price For price or distributor address,
please select your country

LyoVec™

Cat. Codelyec-22
Unit Size18 ml, 360 reactions
Price For price or distributor address,
please select your country

LyoVec™

Description Lyophilized cationic lipid for transfection of mammalian cells
Cat. Codelyec-1
Unit Size10 ml, 200 reactions
Price For price or distributor address,
please select your country

LyoVec™

Description Lyophilized cationic lipid for transfection of mammalian cells
Cat. Codelyec-2
Unit Size20 ml, 400 reactions
Price For price or distributor address,
please select your country
Disclaimer: Our products are provided for research purpose only. Commercial applications may require licensing from third parties.
Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
Copyrights © 2011-2016 InvivoGen. All Rights Reserved. Reproduction of any materials from this site is strictly forbidden without permission for commercial use. Nonprofit use for non-commercial research and educational purposes is permitted, citation should include the URL "www.invivogen.com".