5′ppp-dsRNA
5' triphosphate double stranded RNA (5' ppp-dsRNA)
5’ triphosphate double stranded RNA (5’ ppp-dsRNA) is a synthetic ligand for retinoic acid-inducible protein I (RIG-I). RIG-I is a cytosolic pattern recognition receptor that senses pathogen-associated molecular patterns (PAMPs) on viral RNA and triggers an antiviral immune response by the activation of type-I interferons (IFN-α and -β).
Specifications
Specificity: RIG-I agonist
Working Concentration: 300 ng- 1 μg/ml
Endotoxin level: EndoFit™ (<0.001 EU/µg)
Solubility: 0.1 mg/ml in water
5’ppp-dsrna
5’- pppGCAUGCGACCUCUGUUUGA -3’ (19 mer)
3’- CGUACGCUGGAGACAAACU -5’ (19 mer)
5’ triphosphate double stranded RNA is obtained by hybridization of one 5’ triphosphate single-stranded 19-mer phosphodiester RNA with its complementary strand (non-triphosphatase). Each strand is chemically synthesized by solid-phase synthesis and purified by reverse phase HPLC.
Documents
Contents
• 5’ppp-dsRNA is available in two quantities:
- 25 μg 5’ppp-dsRNA: cat code #tlrl-3prna
- 100 μg (4x25μg) 5’ppp-dsRNA #cat code tlrl-3prna-100
• sterile apyrogenic water, 2 ml with #tlrl-3prna and 10 ml with #tlrl-3prna-100
5’ppp-dsRNA is provided lyophilized and shipped at room temperature. Store lyophilized 5’ppp-dsRNA at -20°C. Lyophilized product is stable 1 year when stored -20°C. Upon resuspension, prepare aliquots and store 5’ppp-dsRNA at -20 ̊C. Resuspended product is stable for 1 month at -20°C. Avoid repeated freeze-thaw cycles.
Description
5’ triphosphate double stranded RNA (5’ ppp-dsRNA) is a synthetic ligand for retinoic acid-inducible protein I (RIG-I). RIG-I is a cytosolic pattern recognition receptor that senses pathogen-associated molecular patterns (PAMPs) on viral RNA and triggers an antiviral immune response by the activation of type-I interferons (IFN-α and -β) [1]. RIG-I shares the identification of the dsRNA structure with another sensor MDA-5 (recognition of poly(I:C)/LyoVec™) and is specifically activated by the uncapped 5’ triphosphate moiety on viral RNA [2]. This triphosphate occurs during viral replication and is absent from most cytosolic self-RNA. A synthetic approach to the exact structure requirement to RIG-I recognition demonstrated that a short blunt double-stranded conformation containing a triphosphate at the 5’ end is required [3-5].
1. Yoneyama M. & Fujita T., 2007. Function of RIG-I-like Receptors in Antiviral Innate Immunity. J. Biol. Chem. 282: 15315-8.
2. Hornung V. et al., 2006. 5'-Triphosphate RNA is the ligand for RIG-I. Science. 314(5801):994-7.
3. Schlee M. et al., 2009. Recognition of 5' triphosphate by RIG-I helicase requires short blunt double-stranded RNA as contained in panhandle of negative-strand virus. Immunity. 17;31(1):25-34.
4. Schmidt a. et al., 2009. 5'- triphosphate RNA requires base-paired structures to activate antiviral signaling via RIG-I. PNAS 106(29):12067-72.
5. Schlee M. & Hartmann G., 2010. The chase for the RIG-I ligand - recent advances. Mol Ther. 18(7):1254-62.
Details
B16-Blue™ IFN α/β cells respond to stimulation with 5'ppp-dsRNA and poly(I:C) when transfected with LyoVec™
ORDERING
5′ppp-dsRNA
| Cat. Code | tlrl-3prna |
|---|---|
| Unit Size | 25 µg |
| Price | For price or distributor address, please select your country |
5′ppp-dsRNA
| Cat. Code | tlrl-3prna-100 |
|---|---|
| Unit Size | 100 µg |
| Price | For price or distributor address, please select your country |
