HEK-Blue™ mMincle Cells

SEAP Reporter 293 cells expressing the murine Mincle gene

HEK-Blue™ mMincle cells stably express the murine Mincle (mMincle) gene, as well as the genes of the Mincle-NF-kB signaling pathway.
They also stably express an NF‑kB-inducible secreted embryonic alkaline phosphatase (SEAP) reporter gene.

These reporter cell lines are activated by Mincle ligands, such as trehalose-6,6-dibehenate (TDB) and heat-killed M. tuberculosis (HKMT). They do not respond to other CLR ligands such as zymosan, a Dectin-1 and TLR2 ligand.

Figures for this product

Response of HEK-Blue™ mMincle cells to CLR agonists


Required Selective Antibiotics: Blasticidin, Puromycin and HEK-Blue™ CLR Selection

Growth medium: DMEM (4.5 g/l glucose), 10% (v/v) fetal bovine serum (FBS), 50 U/ml penicillin, 50 µg/ml streptomycin, 100 µg/ml Normocin™, 2 mM L-glutamine

Guaranteed mycoplasma-free

Shipped on dry ice


1 vial containing 3-7 x 106 cells

100 μl blasticidin (10 mg/ml).

100 μl puromycin (10 mg/ml).

2 ml HEK-Blue™ CLR Selection (250x) (a solution containing several selection antibiotics)

1 ml Normocin™ (50 mg/ml)

1 pouch of HEK-Blue™ Detection (cell culture medium for real-time detection of SEAP)



Quality Control
HEK-Blue™ mMincle cells have been stimulated by various pathogen recognition receptor (PRR) agonists. As expected, Mincle agonists induced the production of SEAP. The cell surface expression of murine Mincle (mMincle) in this cell line has been validated using fluorescence-activated cell sorting (FACS). The stability of this cell line for 20 passages following thawing has been verified.

Specificity of HEK-Blue™ mMincle cells
As their parental cell line, HEK293 cells, express endogenous levels of TLR3, TLR5 and NOD1, HEK-Blue™ mMincle cells will respond to TLR3, TLR5 and NOD1 agonists, such as poly(I:C), flagellin and C12‑iE‑DAP, respectively. In order to identify Mincle-specific responses, we recommend to use HEK-Blue™ Null1-v cells as a control cell line.


Recent articles using InvivoGen HEK-Blue™ mMincle Cells


HEK-Blue™ mMincle Cells

Description Murine Mincle / NF-κB / SEAP reporter HEK293 cells
Cat. Codehkb-mmcl
Unit Size3-7 x 10e6 cells
Price For price or distributor address,
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Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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