HEK-Blue™ hDectin-1b Cells

SEAP Reporter 293 cells expressing the human Dectin-1b gene

HEK-Blue™ hDectin-1b cells have been engineered to express a secreted alkaline phosphatase (SEAP) in response to stimulation by Dectin-1 ligands

Dectin-1 is a C-type lectin receptor (CLR) that recognizes β-glucans, glucose polymers found in the cell walls of fungi, and thus plays an important role in antifungal innate immunity. Dectin-1 is alternatively spliced into 2 major isoforms: a full-length A isoform and a ‘stalkless’ B isoform, which do not induce the same response to soluble and particulate β-glucans [1].

HEK-Blue™ hDectin-1b cells are derived from the human embryonic kidney HEK293 cells which do not naturally express Dectin-1. We have engineered HEK-Blue™ cells that stably express high levels of human Dectin-1b isoform and genes involved in the Dectin-1/NF-κB/SEAP signaling pathway. Levels of SEAP are readily measurable in real-time by a simple colorimetric assay with HEK-Blue™ Detection or QUANTI-Blue™ . In contrast to cells expressing the human Dectin‑1a gene, HEK‑Blue™ hDectin‑1b cells do not respond to soluble β-glucans, such as laminarin and WGP soluble.

Find out more on Dectin-1 & β-glucans.

Find out more on Dectin-1 & inflammasomes.

1. Heinsbroek, S.E, et al, 2006. Expression of functionally different Dectin-1 isoforms by murine macrophages. J Immunol. 176: 5513.

Figures for this product

Evaluation of NF-κB responses to Dectin-1 ligandsInhibition of HEK-Blue™ hDectin-1b responses by Laminarin and WGP soluble


Required Selective Antibiotics: Puromycin and HEK-Blue™ CLR Selection

Growth medium: DMEM (4.5 g/l glucose), 10% (v/v) fetal bovine serum (FBS), 50 U/ml penicillin, 50 µg/ml streptomycin, 100 µg/ml Normocin™, 2 mM L-glutamine

Guaranteed mycoplasma-free

Shipped on dry ice


1 vial containing 3-7 x 106 cells

100 μl puromycin (10 mg/ml).

2 ml HEK-Blue™ CLR Selection (250x) (a solution containing several selection antibiotics)

1 ml Normocin™ (50 mg/ml)

1 pouch of HEK-Blue™ Detection (cell culture medium for real-time detection of SEAP)


Quality Control
HEK-Blue™ hDectin-1b cells have been stimulated by various pathogen recognition receptor (PRR) agonists. As expected, Dectin-1 agonists induced the production of SEAP. The cell surface expression of human Dectin-1b (hDectin-1b) in this cell line has been validated using fluorescence-activated cell sorting (FACS). The stability of this cell line for 20 passages following thawing has been verified.

Specificity of HEK-Blue™ hDectin-1b cells:
As their parental cell line, HEK293 cells, express endogenous levels of TLR3, TLR5 and NOD1, HEK-Blue™ hDectin-1b cells will respond to TLR3, TLR5 and NOD1 agonists, such as poly(I:C), flagellin and C12‑iE‑DAP, respectively.
In order to identify Dectin-1-specific responses, we recommend to use HEK-Blue™ Null1-v cells as a control cell line.


Recent articles using InvivoGen HEK-Blue™ hDectin-1b Cells


HEK-Blue™ hDectin-1b cells

Description human Dectin-1b-expressing HEK 293 cells
Cat. Codehkb-hdect1b
Unit Size3-7 x 10e6 cells
Price For price or distributor address,
please select your country
Disclaimer: Our products are provided for research purpose only. Commercial applications may require licensing from third parties.
Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
Copyrights © 2011-2016 InvivoGen. All Rights Reserved. Reproduction of any materials from this site is strictly forbidden without permission for commercial use. Nonprofit use for non-commercial research and educational purposes is permitted, citation should include the URL "www.invivogen.com".