HEK-Blue™ mDectin-2 Cells

SEAP Reporter 293 cells expressing the mouse Dectin-2 gene

HEK-Blue™ mDectin-2 cells stably express the mouse Dectin-2 gene, as well as the genes of the Dectin-2 signaling pathway. They also stably express an NF-kB-inducible secreted embryonic alkaline phosphatase (SEAP) reporter gene.
These reporter cell lines are activated by Dectin-2 ligands. They do not respond to other CLR ligands, such as trehalose-6,6-dibehenate (TDB), a Mincle ligand.

Levels of SEAP can be easily determined with HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP. SEAP activity can also be assessed using the alkaline phosphatase detection reagent, QUANTI-Blue™.

Figures for this product

HEK-Blue™ mDectin-2 cells


Required Selective Antibiotics: Blasticidin, Puromycin and HEK-Blue™ CLR Selection

Growth medium: DMEM (4.5 g/l glucose), 10% (v/v) fetal bovine serum (FBS), 50 U/ml penicillin, 50 µg/ml streptomycin, 100 µg/ml Normocin™, 2 mM L-glutamine

Guaranteed mycoplasma-free

Shipped on dry ice


1 vial containing 3-7 x 106 cells

100 μl blasticidin (10 mg/ml).

100 μl puromycin (10 mg/ml).

2 ml HEK-Blue™ CLR Selection (250x) (a solution containing several selection antibiotics)

1 ml Normocin™ (50 mg/ml)

1 pouch of HEK-Blue™ Detection (cell culture medium for real-time detection of SEAP)


Quality Control
HEK-Blue™ mDectin-2 cells have been stimulated by various pathogen recognition receptor (PRR) agonists. As expected, Dectin-2 agonists induced the production of SEAP. The cell surface expression of murine Dectin-2 (mDectin-2) in this cell line has been validated using fluorescence-activated cell sorting (FACS). The stability of this cell line for 20 passages following thawing has been verified.

Specificity of HEK-Blue™ mDectin-2 cells
As their parental cell line, HEK293 cells, express endogenous levels of TLR3, TLR5 and NOD1, HEK-Blue™ mDectin-2 cells will respond to TLR3, TLR5 and NOD1 agonists, such as poly(I:C), flagellin and C12‑iE‑DAP, respectively. In order to identify Dectin-2-specific responses, we recommend to use HEK-Blue™ Null1-v cells as a control cell line.


Recent articles using InvivoGen HEK-Blue™ mDectin-2 Cells


HEK-Blue™ mDectin-2 Cells

Description Murine Dectin-2 / NF-κB / SEAP reporter HEK293 cells
Cat. Codehkb-mdect2
Unit Size3-7 x 10e6 cells
Price For price or distributor address,
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Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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