2’3’-cGAMP VacciGrade™

Cyclic [G(2’,5’)pA(3’,5’)p] - STING ligand

2’3’-cGAMP (cyclic [G(2’,5’)pA(3’,5’)p]) is a cyclic dinucleotide (CDN) produced in mammalian cells by cGAS (cGAMP synthase) in response to double-stranded DNA in the cytoplasm. 2’3’-cGAMP is also referred to as “noncanonical” cGAMP due to the presence of the atypical 2’-5’ phosphodiester linkage between the guanosine and the adenosine. Structural and functional studies revealed that noncanonical 2’3’-cGAMP is distinct from the canonical 3’3’-cGAMP produced by bacteria [1, 2].

CDNs are a relatively new class of adjuvants that have been shown to increase vaccine potency [3]. CDNs activate innate immunity by directly binding the endoplasmic reticulum-resident receptor STING (stimulator of interferon genes), activating a signaling pathway that induces the expression of interferon-β (IFN-β) and also nuclear factor-κB (NF-κB) dependent inflammatory cytokines.

Recently, it has been reported that 2′3′-cGAMP is an effective adjuvant that boosts the production of antigen-specific antibodies and T cell responses in mice [4].

2’3’-cGAMP VacciGrade™ is a preclinical grade preparation of the cyclic dinucleotide 2’3’-cGAMP.

2’3’-cGAMP VacciGrade™ is for research use only, not for use in humans.

Figures for this product

IRF INDUCTION (Lucia luciferase reporter)NF-kB INDUCTION (SEAP reporter)


Description: STING agonist VacciGrade™
Synonym: Cyclic [G(2’,5’)pA(3’,5’)p], cyclic GMP-AMP; c-GpAp sodium salt
CAS number: 1441190-66-4
Formula: C20H22N10O13P2 .2Na
Molecular weight: 718.38
Purity: ≥ 95% by LC/MS
Solubility: 50 mg/ml in physiological water
Quality: Sterile
Endotoxin Level: Lower than 0.005 EU/μg
Biological activity: Assessed by measuring induction of the interferon pathway in THP1-Blue™ ISG cells


• 1 mg (2 x 500 µg) lyophilized 2’3’-cGAMP VacciGrade™
• 10 ml sterile endotoxin-free physiological water (NaCl 0.9%)

2’3’-cGAMP VacciGrade™ is shipped at room temperature and should be stored at -20°C.


2’3’-cGAMP VacciGrade™ is a preclinical grade preparation of the cyclic dinucleotide 2’3’-cGAMP. It is prepared under strict aseptic conditions and is tested for the presence of endotoxins. 2’3’-cGAMP VacciGrade™ is guaranteed sterile. Its endotoxin level is lower than 0.005 EU/μg.


 1. Diner E. et al., 2013. The Innate Immune DNA Sensor cGAS Produces a Noncanonical Cyclic Dinucleotide that Activates Human STING. Cell Rep. 3(5):1355-61.
2. Gao P. et al., 2013. Cyclic [G(2',5')pA(3',5')p] is the metazoan second messenger produced by DNA-activated cyclic GMP-AMP synthase. Cell. 153(5):1094-107.
3. Dubensky TW. et al., 2013. Rationale, progress and development of vaccines utilizing STING-activating cyclic dinucleotide adjuvants. Therapeutic Advances in Vaccines 1(4): 131-143.
4. Li XD. et al., 2013. Pivotal roles of cGAS-cGAMP signaling in antiviral defense and immune adjuvant effects. Science. 341(6152):1390-4.


Recent articles using InvivoGen 2’3’-cGAMP VacciGrade™


2’3’-cGAMP VacciGrade™

Description STING ligand - Cyclic [G(2’,5’)pA(3’,5’)p]
Cat. Codevac-nacga23
Unit Size1 mg
Price For price or distributor address,
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Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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