CL413 VacciGrade™

Dual TLR2 & TLR7 agonist

CL413 is a chimeric molecule that co-activates the surface receptor TLR2 and the endosomal receptor TLR7. It is composed of 8-hydroxyadenine (TLR7 ligand) conjugated to the terminal acid function of Pam2CSK4 (TLR2 ligand). CL413 is water-soluble.

This chimeric compound displays strong adjuvant activity. In mice, administration of OVA with CL413 leads to a significant increase of anti-OVA IgG titers in sera. The predominant IgG subclass is IgG1, a Th2-associated isotype. The IgG2a subclass, a Th1-associated isotype, is also significantly increased resulting in a decreased IgG1/IgG2a ratio.

CL413 VacciGrade™ is a high-quality pre-clinical grade.
CL413 VacciGrade™​ is for research use only, and not for human or veterinary use.

Specifications

Description: TLR2 & TLR7  agonist VacciGrade™

Polarization of adaptive immune response: Th1 & Th2 responses

Synonym: S-(2,3-bis(palmitoyloxy)-(2RS)propyl)-(R)-cysteinyl-(S)-seryl-(S)-lysyl-(S)-lysyl-(S)-lysyl-(S)-lysyl 4-((6-amino-2-(butylamino)-8-hydroxy-9H-purin-9-yl)methyl)aniline

Formula: C₈₁H₁₄₅N₁₇O₁₂S

Molecular weight: 1581 g/mol

Solubility: 1 mg/ml in physiological water

Working concentration: 20 - 50 µg/mouse

Quality control:

• Sterility guaranteed
• The absence of bacterial contamination (lipoproteins & endotoxins) has been confirmed using HEK-Blue™ TLR4 cells
• Endotoxin level < 5 EU/mg (determined using the HEK-Blue™ LPS Detection Kit 2)

Contents

• 5 mg CL413 VacciGrade™ 
• 10 ml of  sterile endotoxin-free physiological water (NaCl 0.9%)

CL413 VacciGrade ™ is shipped at room temperature.

Store lyophilized product at -20 °C.

Lyophilized product is stable for 1 year at -20 °C.

VacciGrade™

VacciGrade™ is a high-quality pre-clinical grade. VacciGrade™ products are filter-sterilized (0.2 µm) and filled under strict aseptic conditions in a clean room*. The absence of bacterial contamination is assessed by a sterility test using a pharmacopeia-derived assay. The level of bacterial contaminants (endotoxins and lipoproteins) in each lot is verified using a LAL assay and/or a TLR2 and TLR4 reporter assay.
*Except for LPS VacciGrade™, which is prepared in a laminar flow hood dedicated to LPS.

Details

Chemical structure of CL413:

InvivoGen has developed a series of novel molecules designed to induce potent immune responses through the combined activation of several pattern recognition receptors (PRRs) that trigger different innate immune signaling pathways.

These molecules are agonists for TLR2, TLR7 or both. Agonists that activate TLR2 are derived from the well-established TLR2 ligand, Pam2CSK4, and those recognized by TLR7 are derived from the 8-hydroxyadenine derivative CL264, a TLR7 agonist recently developed by InvivoGen.

TLR2 and TLR7 are two PRRs with distinct characteristics. TLR2 is a cell surface receptor expressed by many cell types, while TLR7 is an endosomal receptor expressed predominantly in plasmacytoid dendritic cells (pDC) and to a lesser extent in B cells.

TLR2 signaling triggers the NF-kB pathway and the production of pro-inflammatory cytokines, such as TNF-α, whereas TLR7 signaling induces mainly the IRF pathway and the production of IFN-α.