Specifications

Specificity: Th1/Th2 response

Working concentration: 1 -10 μg/mouse

Endotoxin level: <0.005 EU/μg

Solubility: 100 µg/ml in physiological water

Documents

• TDS Flagellin FliC VacciGrade : 50 µg (vac-fla)
• MSDS Flagellin FliC VacciGrade : 50 µg (vac-fla)

Contents

• 50 mg of sterile lyophilized Flagellin FliC VacciGrade™
• 10 ml sterile endotoxin-free physiological water (NaCl 0.9%)

Flagellin FliC VacciGrade™ is shipped at room temperature and should be stored at -20°C. Lyophilized product is stable 1 year at -20°C when properly stored. Upon resuspension, prepare aliquots of Flagellin FliC VacciGrade™ and store at -20˚C. Resuspended product is stable 6 months at -20°C. Avoid repeated freeze-thaw cycles.

Description

Flagellin FliC is a recombinant flagellin protein encoded by the fliC gene from Salmonella typhimurium. Bacterial flagellin, a TLR5 ligand, is a potent T-cell antigen and has potential as a vaccine adjuvant. Unlike other TLR agonists, flagellin tends to produce mixed Th1 and Th2 responses rather than strong Th1 responses [1]. It has been demonstrated that flagellin can act as a potent adjuvant in flu vaccines [2, 3]. Furthermore, flagellin can also signal through the NLRC4 inflammasome [4], although it is not known whether this pathway contributes to the adjuvant activity of flagellin.

1. Huleatt J. et al., 2007. Vaccination with recombinant fusion proteins incorporating Toll-like receptor ligands induces rapid cellular and humoral immunity. Vaccine 25(4): 763-75.
2. Mbow ML. et al., 2010. New adjuvants for human vaccines. Curr Opin Immunol. 22(3):411-6.
3. Skountzou I. et al., 2010. Salmonella flagellins are potent adjuvants for intranasally administered whole inactivated influenza vaccine. Vaccine 28(4): 4103-12.
4. Miao EA. & Warren SE., 2010. Innate immune detection of bacterial virulence factors via the NLRC4 inflammasome. J Clin Immunol. 30(4): 502-6