Alhydrogel® 2%

Vaccine Adjuvant: Aluminium hydroxide gel

Alhydrogel® 2%, referred to as alum, is an aluminium hydroxide wet gel suspension. Alum induces a Th2 response by improving the attraction and uptake of antigen by antigen-presenting cells (APCs). It can also activate innate immunity pathways triggered by pattern recognition receptors (PRRs).

Alhydrogel® is sterilized by autoclavation and aseptically filled. InvivoGen only sells products for research use
Alhydrogel® is a trademark of Brenntag Biosector.

Please note that our standard-sized Alhyydrogel® (250 mL) is currently on back order until February 2015; however, we are currently offering a larger size (Unit Size : 1 L., Cat. Code : Vac-alu-1000).

Specifications

Specificity: Th2 response
Working concentration: 1:9 - 1:1 (Alhydrogel:antigen)
CAS number: 21645-51-2
Quality: Sterile
Aluminium content: 9.0 – 11.0 mg/ml
Appearance: White gelatinous precipitate
Quality control:
- Alhydrogel® 2% is tested for pyrogenicity and sterility.
- Adjuvanticity of Alhydrogel® 2% was evaluated by assessing the levels of total mouse IgGs (mIgGs) and the mIgG1 and mIgG2 isotypes after two subcutaneous injections of EndoFit™ Ovalbumin/Alhydrogel® 2% (1:1, v/v) in mice. Results were compared to mice which received EndoFit™ Ovalbumin alone.

Contents

• 250 ml Alhydrogel 2%, provided as a ready-to-use, sterile wet gel suspension

Alhydrogel 2% is shipped at room temperature and should be stored at room temperature. Product is stable for 6 months. DO NOT FREEZE.

Description

Alhydrogel 2% is an aluminium hydroxide (referred to as alum) wet gel suspension. Alum acts primarily through the formation of a depot at the injection site, enabling enhanced antigen availability, activation of antigen presenting cells (APCs) and uptake by immune cells [1]. It can also activate innate immunity pathways triggered by pattern recognition receptors (PRRs). The NLR pathway has been recently described as an important mechanism in alum adjuvancy [2]. Alum has been shown to activate the NLRP3 inflammasome although its role in alum-induced antibody response is controversial [3].

1. Morefield GL. et al., 2005. Role of aluminum-containing adjuvants in antigen internalization by dendritic cells in vitro. Vaccine 23(13): 1588-95.
2. Li H.  et al., 2008. Cutting edge: Inflammasome activation by alum and alum's adjuvant effect are mediated by NLRP3. J Immunol. 181(1):17-21.
3. Franchi L. & Nuñez G., 2008. The Nlrp3 inflammasome is critical for aluminium hydroxide-mediated IL-1beta secretion but dispensable for adjuvant activity. Eur. J. Immunol. 38(8):2085-9  

Citations

Recent articles using InvivoGen Alhydrogel® 2%

ORDERING

Alhydrogel® 2%

Description Aluminium hydroxide gel
Cat. Code vac-alu-1000
Unit Size 1 L
Price For price or distributor address,
please select your country

Alhydrogel® 2%

Description Aluminium hydroxide gel
Cat. Code vac-alu-250
Unit Size 250 ml
Price For price or distributor address,
please select your country
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Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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