Muramyl dipeptide (MDP) is the minimal bioactive peptidoglycan motif common to all bacteria, the essential structure required for adjuvant activity in vaccines. MDP has been shown to be recognized by NOD2, but not TLR2, nor TLR2/1 or TLR2/6 associations [1,2]. In an attempt to enhance the protective activity against bacterial infection, numerous derivatives of MDP have been synthesized. Among them, L18-MDP, a 6-O-acyl derivative with a stearoyl fatty acid, showed the highest activity . In HEK-Blue™ NOD2 cells, L18-MDP was 10 times more efficient than MDP to induce NF-κB activation.
Specificity: NOD2 agonist
Working concentration: 1 - 100 ng/ml
Endotoxin level: <0.125 EU/mg
Solubility: 1 mg/ml in water
Molecular weight: 758.94
• 1 mg L18-MDP
• 2 ml sterile endotoxin-free water
L18-MDP is provided as a sterile white lyophilized powder and shipped at room temperature. Store at -20°C. Upon resuspension, L18-MDP should be aliquoted and stored at -20°C. Product is stable 6 months at -20°C when properly stored. Avoid repeated freeze-thaw cycles.
1. Girardin SE. et al., 2003. Nod2 is a general sensor of peptidoglycan through muramyl dipeptide (MDP) detection. J Biol Chem. 278(11):8869-72.
2. Inohara N. et al., 2003. Host recognition of bacterial muramyl dipeptide mediated through NOD2. Implications for Crohn's disease.J Biol Chem. 278(8):5509-12.
3. Matsumoto K. et al., 1981. Stimulation of nonspecific resistance to infection induced by 6-O-acyl muramyl dipeptide analogs in mice. Infect Immun. 32(2):748-58.
Recent articles using InvivoGen L18-MDP
- 2014 - PNAS, 111(25):E2559-66.
Nod/Ripk2 signaling in dendritic cells activates IL-17A-secreting innate lymphoid cells and drives colitis in T-bet-/-.Rag2-/- (TRUC) mice.
Ermann J, Staton T, Glickman JN, de Waal Malefyt R, Glimcher LH.
- 2013 - Infect Immun., 81(10):3855-64.
Combined stimulation of Toll-like receptor 5 and NOD1 strongly potentiates activity of NF-κB, resulting in enhanced innate immune reactions and resistance to Salmonella enterica serovar Typhimurium infection.
Tukhvatulin AI, Gitlin I, Shcheblyakov DV, Artemicheva NM, Burdelya LG, Shmarov MM, Naroditsky BS, Gudkov AV, Gintsburg AL, Logunov DY.
- 2012 - J Immunol., 189(1):318-327
Gadolinium Compounds Signaling through TLR 4 and TLR 7 in Normal Human Macrophages: Establishment of a Proinflammatory Phenotype and Implications for the Pathogenesis of Nephrogenic Systemic Fibrosis.
Wermuth PJ, Jimenez SA