Ramos-Blue™ KD-MyD Cells

Ramos-Blue™ KD-MyD (MyD88 knockdown) is a B lymphocyte cell line specifically designed to monitor MyD88-mediated signaling.

Ramos-Blue™ KD-MyD were obtained by stable integration of an NF-κB/AP-1-inducible SEAP (secreted embryonic alkaline phosphate) reporter gene and stable knowndown of the MyD88 gene. Due to the knockdown of the MyD88 gene, Ramos-Blue™ KD-MyD do not respond or respond weakly to the activation of receptors which signaling is dependent on MyD88, such as TLR7 and TLR9. However, they are still responsive to TLR3 which signals through TRIF independently of MyD88.

When stimulated, they produce SEAP in the supernatant that can be readily monitored using QUANTI-Blue™, a medium that turns purple/blue in the presence of SEAP.
Ramos-Blue™ KD-MyD cells derive from a human Burkitt’s lymphoma which is negative for Epstein Barr virus.

Figures for this product

MyD88 dependent and independent responses in Ramos-Blue™ and Ramos-Blue™-defMyD


Antibiotic resistance: Hygromycin B and Zeocin™

Growth Medium: IMDM (Iscove’s Modified Dulbecco’s Medium), 2 mM L-glutamine, 10% v/v heat-inactivated fetal bovine serum (30 min at 56°C), 100 µg/ml Normocin™, Pen-Strep (50 U/ml-50 μg/ml).

Guaranteed mycoplasma-free

Shipped on dry ice


1 vial containing 3-7 x 106 cells

100 μl Zeocin™ (100 mg/ml)

100 μl Hygromycin B Gold (ultrapure hygromycin B; 100 mg/ml)

1 ml Normocin™ (50 mg/ml)

1 pouch of QUANTI-Blue™ (100 ml)


Ramos-Blue™ KD-MyD Cells

Description NF-κB/AP-1 SEAP reporter B lymphocytes with MyD88 knockdown
Cat. Coderms-kdmyd
Unit Size3-7 x 10e6 cells
Price For price or distributor address,
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Note that the sequence of available ORFs provided by InvivoGen can differ from a given reference Genbank record due to genetic variations and/or alternative splicing. Customers should verify that the version of a gene sold by InvivoGen is suitable for the customer needs.
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